Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations.
Fractionation of Pneumocystis carinii developmental stages by counterflow centrifugal elutriation and sequential filtrations.
复制标题
通过逆流离心淘洗和顺序过滤对卡氏肺囊虫发育阶段进行分级。
DOI：
10.1007/bf00932616
复制
发表时间：
1994
影响因子：
2
通讯作者：
Walzer,PD
中科院分区：
文献类型：
--
作者：
DeStefano,JA;Foy,JM;Sullivan,DW;Dawes,SM;Cushion,MT;Babcock,GF;Sleight,RG;vanHalbeek,H;Walzer,PD
Immunomagnetic sorting, sequential filtrations, and counterflow centrifugal elutriation were compared for their ability to obtain enriched populations ofPneumocystis cariniidevelopmental stages from infected rat-lung homogenates. Elutriation combined with sequential filtrations resulted in highly (>95%) enriched populations ofP. cariniicysts and trophozoites with excellent viability. This approach offers advantages over previously described methods of obtaining enrichedP. cariniicell populations and should have important applications to research on this organism.
