Effects of elevated CO(2) levels on lung immune response to organic dust and lipopolysaccharide.
Effects of elevated CO(2) levels on lung immune response to organic dust and lipopolysaccharide.
复制标题
DOI：
10.1186/s12931-021-01700-4
复制
发表时间：
2021-04-09
影响因子：
5.8
通讯作者：
Kirychuk S
中科院分区：
文献类型：
--
作者：
Schneberger D;Pandher U;Thompson B;Kirychuk S
有机粉尘水平升高的工作场所，如动物饲料谷仓，通常也会有二氧化碳等气体水平升高。暴露在这种复杂环境中的工人经常会出现呼吸影响，这可能是由于多种呼吸因素的组合造成的。我们研究了二氧化碳对炎症介质脂多糖(LPS)和有机粉尘联合暴露的小鼠肺天然免疫反应的影响。我们评估了建筑建议限值(1000ppm)和暴露限值(5000ppm)下的二氧化碳水平。小鼠鼻腔内注入粉尘提取物或脂多糖，并立即放入室内空气持续流动的室内(avg。430(ppm二氧化碳)、1000(Ppm)或5000(Ppm)二氧化碳浓缩空气。结果显示，接触有机粉尘后，当二氧化碳浓度为1000ppm时，炎症反应降低，而二氧化碳浓度为5000ppm时，反应增加。相反，添加低至1000ppm二氧化碳的内毒素倾向于抑制几种炎症标志物。在大多数情况下，盐水处理的动物几乎没有随着二氧化碳暴露而发生变化，尽管存在一些mRNA水平的变化。这表明，低至1000ppm的二氧化碳能够改变对脂多糖和有机粉尘提取物的先天免疫反应，但每种反应都以不同的方式改变。
Workplaces with elevated organic dust levels such as animal feed barns also commonly have elevated levels of gasses, such as CO2. Workers exposed to such complex environments often experience respiratory effects that may be due to a combination of respirable factors. We examined the effects of CO2 on lung innate immune responses in mice co-exposed to the inflammatory agents lipopolysaccharide (LPS) and organic dust. We evaluated CO2 levels at the building recommended limit (1000 ppm) as well as the exposure limit (5000 ppm). Mice were nasally instilled with dust extracts or LPS and immediately put into chambers with a constant flow of room air (avg. 430 ppm CO2), 1000 ppm, or 5000 ppm CO2 enriched air. Results reveal that organic dust exposures tended to show decreased inflammatory responses with 1000 ppm CO2 and increased responses at 5000 ppm CO2. Conversely, LPS with addition of CO2 as low as 1000 ppm tended to inhibit several inflammatory markers. In most cases saline treated animals showed few changes with CO2 exposure, though some changes in mRNA levels were present. This shows that CO2 as low as 1000 ppm CO2 was capable of altering innate immune responses to both LPS and organic dust extracts, but each response was altered in a different fashion.
登录
查看更多内容
DOI：
10.1165/ajrcmb.15.1.8679226
发表时间：
1996-07-01
影响因子：
6.4
作者：
Nicholson, WJ;Slight, J;Donaldson, K
通讯作者：
Donaldson, K
DOI：
10.1165/rcmb.2010-0427oc
发表时间：
2011-10-01
影响因子：
6.4
作者：
Poole, Jill A.;Wyatt, Todd A.;Romberger, Debra J.
通讯作者：
Romberger, Debra J.
DOI：
10.1152/ajpgi.00177.2010
发表时间：
2010-10-01
影响因子：
4.5
作者：
Orlichenko, Lidiya S.;Behari, Jaideep;Singh, Vijay P.
通讯作者：
Singh, Vijay P.
影响因子：
158.5
作者：
HOGE, CW;REICHLER, MR;BREIMAN, RF
通讯作者：
BREIMAN, RF
影响因子：
5.1
作者：
PATTON, LM;SAGGART, BS;REPINE, JE
通讯作者：
REPINE, JE