Ovarian intrabursal administration of transforming growth factor beta 1 inhibits follicle rupture in gonadotropin-primed mice.
Ovarian intrabursal administration of transforming growth factor beta 1 inhibits follicle rupture in gonadotropin-primed mice.
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卵巢囊内注射转化生长因子β1可抑制促性腺激素引发的小鼠卵泡破裂。
作者：
S. Juneja;N. Chegini;R. Stan Williams;G. Ksander
我们推测，各种类型的卵巢细胞过度表达和/或激活潜伏性转化生长因子β（TGF β）可能导致排卵和受精障碍。为了验证这一假设，将1至500 ng的活性TGF β囊内注射到促性腺激素致敏小鼠的卵巢中，并测定卵子回收率和受精率。此外，内源性TGF β和TGF β I型和II型受体的存在和细胞分布在TGF β 1处理组和未处理组的卵巢组织中进行化学测定。从成对处理或单独用1至10 ng/卵巢的TGF β 1处理的卵巢中回收的每个卵巢的卵子总数与对照组相似，而从成对或单独用50或100 ng/卵巢的TGF β 1处理的卵巢中回收的卵子总数显著低于各自对照组的数量（p < 0.05，0.001）。从用200或500 ng/卵巢的TGF β 1成对或单独处理的卵巢中每个卵巢回收的卵子数量与从用100 ng/卵巢的TGF β 1处理的卵巢中获得的卵子数量相似。与未处理卵巢的卵子相比，从50、100、200和500 ng/卵巢的TGF β 1处理的卵巢中回收的卵子的体外受精率较低。组织学上，TGF β 1处理的卵巢含有大量未破裂的卵泡，而未处理的卵巢含有大量黄体。免疫组化显示，内源性TGF β 1和TGF β 2定位于卵泡膜、颗粒层和黄体细胞，在TGF β 1处理的卵巢和对照组中，其强度或分布无显著差异。膜细胞是TGF β免疫反应蛋白的主要部位。TGF β I型和II型受体也存在于这些细胞中，它们的相对免疫反应强度大大降低，特别是在TGF β 1处理的卵巢颗粒细胞与对照组相比。这些结果支持了我们的假设，并表明TGF β在卵泡发育、卵母细胞成熟和排卵过程中起着重要的调节作用。
We hypothesized that over-expression and/or activation of latent transforming growth factor betas (TGF betas) by various ovarian cell types may lead to disturbances in ovulation and fertilization. To test this hypothesis, active TGF beta ranging from 1 to 500 ng was administered intrabursally into the ovaries of gonadotropin-primed mice, and the rates of ovum recovery and fertilization were determined. Furthermore, the presence and cellular distribution of endogenous TGF betas and TGF beta type I and type II receptors were determined immunohistochemically in the ovarian tissues of TGF beta 1-treated and untreated groups. The total number of ova recovered per ovary from ovaries treated as pairs or treated singly with TGF beta 1 at 1 to 10 ng/ovary was similar to that from controls, whereas the number recovered from ovaries treated as pairs or singly with 50 or 100 ng of TGF beta 1 per ovary was significantly lower than the number from respective controls (p < 0.05, 0.001). The number of ova recovered per ovary from ovaries treated as pairs or singly with TGF beta 1 at 200 or 500 ng/ovary was similar to the number of ova obtained from ovaries treated with TGF beta 1 at 100 ng/ovary. The rate of in vitro fertilization was low in ova recovered from ovaries treated with 50, 100, 200, and 500 ng/ovary of TGF beta 1, compared to that in ova from untreated ovaries. Histologically, the TGF beta 1-treated ovaries contained large numbers of unruptured follicles, whereas untreated ovaries contained large numbers of corpora lutea. Immunohistochemically, the endogenous TGF beta 1 and TGF beta 2 was localized in theca, granulosa, and luteal cells, without a substantial difference in intensity or distribution, in both TGF beta 1-treated ovaries and in controls. Theca cells were the primary site of immunoreactive TGF beta protein. TGF beta type I and type II receptors were also present in these cells, and their relative immunoreactive intensity was considerably reduced, particularly in granulosa cells in TGF beta 1-treated ovaries compared to controls. The results support our hypothesis and suggest that TGF betas play an important regulatory role in follicular development, oocyte maturation, and the ovulatory process.
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影响因子：
4.8
作者：
DODSON, WC;SCHOMBERG, DW
通讯作者：
SCHOMBERG, DW
影响因子：
--
作者：
DOWNS, SM;DANIEL, SAJ;EPPIG, JJ
通讯作者：
EPPIG, JJ
人颗粒黄体细胞和卵丘细胞表达转化生长因子-β 1 型和 2 型 mRNA。
DOI：
10.1210/jcem.74.2.1370508
发表时间：
1992
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Mulheron,GW;Bossert,NL;Lapp,JA;Walmer,DK;Schomberg,DW
通讯作者：
Schomberg,DW
转化生长因子-β 1 抑制卵巢雄激素的产生：基因表达、细胞定位、机制和作用位点。
影响因子：
4.8
作者：
Hernandez,ER;Hurwitz,A;Payne,DW;Dharmarajan,AM;Purchio,AF;Adashi,EY
通讯作者：
Adashi,EY
大鼠颗粒细胞表达转化生长因子-β 2 型信使核糖核酸，该核糖核酸可在体外受卵泡刺激素调节。
影响因子：
4.8
作者：
Mulheron,GW;Schomberg,DW
通讯作者：
Schomberg,DW