The Effect of Carbon Ion Beam Irradiation for Hypoxia-Mediated Invasion of Glioblastoma
The Effect of Carbon Ion Beam Irradiation for Hypoxia-Mediated Invasion of Glioblastoma
复制标题
碳离子束照射对缺氧介导的胶质母细胞瘤侵袭的影响
作者：
Minami K.;Koizumi M.;Matsuura M.;et al.
胶质母细胞瘤具有侵袭性和放射抵抗性，是最致命的恶性肿瘤之一。不幸的是，据报道光子照射和缺氧条件分别增强癌细胞的侵袭性。本研究的目的是阐明碳离子束对胶质母细胞瘤细胞侵袭潜能的影响。以1%存活率剂量测定的A172细胞的相对生物学效应为2.22。x照射和缺氧分别促进了A172和T98G细胞的侵袭和迁移，而碳离子束抑制了缺氧增强的这两种能力。GTP-RhoA（活性形式）在0.5 Gy x射线照射下增加。另一方面，1 Gy和4 Gy的碳离子束使RhoA失活。碳离子束辐照通过RhoA的失活抑制胶质母细胞瘤细胞的侵袭和迁移。此外，碳离子束还抑制了缺氧所增强的这些能力。
Glioblastoma is one of the most fatal malignant tumors because of its invasiveness and radioresistance. Unfortunately photon irradiation and hypoxic condition are reported to enhance cancer cells’ aggressiveness respectively. The purpose of this study is to clarify the effect of carbon ion beam on invasive potential of glioblastoma cells. Relative Biological Effectiveness of A172 cells measured by the dose for 1% survival fraction was 2.22. X-irradiation and hypoxic condition promoted invasion and migration of A172 and T98G cells respectively, while carbon ion beam suppressed both the two capabilities enhanced by hypoxia. GTP-RhoA (active form) was increased by 0.5 Gy of X-irradiation. On the other hand RhoA was inactivated by 1 and 4 Gy of carbon ion beam. Carbon ion beam irradiation suppressed glioblastoma cells’ invasion and migration via inactivation of RhoA. Furthermore carbon ion beam also inhibited these abilities enhanced by hypoxia.