Effects of Recombinant Cholera Toxin B Subunit (rCTB) on Cellular Immune Responses: Enhancement of Delayed‐Type Hypersensitivity Following Intranasal Co‐Administration of Mycobacterium bovis‐BCG with rCTB
Effects of Recombinant Cholera Toxin B Subunit (rCTB) on Cellular Immune Responses: Enhancement of Delayed‐Type Hypersensitivity Following Intranasal Co‐Administration of Mycobacterium bovis‐BCG with rCTB
复制标题
重组霍乱毒素 B 亚基 (rCTB) 对细胞免疫反应的影响：牛分枝杆菌-BCG 与 rCTB 鼻内联合给药后迟发型超敏反应增强
DOI：
10.1111/j.1348-0421.2004.tb03536.x
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发表时间：
2004
影响因子：
2.6
通讯作者：
N. Goto
中科院分区：
文献类型：
--
作者：
J. Maeyama;M. Isaka;Y. Yasuda;K. Matano;K. Morokuma;K. Ohkuma;K. Tochikubo;Saburo Yamamoto;N. Goto
重组霍乱毒素B亚单位（rCT B）是一种安全有效的粘膜佐剂。为了深入了解rCTB佐剂作用的机制，在鼻内给予牛分枝杆菌卡介菌（BCG）（含和不含rCTB）后，检查了rCTB对小鼠和豚鼠细胞介导的免疫应答的影响。与仅给予BCG相比，BCG和rCTB鼻内联合给药增强了豚鼠皮肤反应和小鼠足垫肿胀反应对纯化蛋白衍生物（PPD）的迟发型超敏反应。此外，在存在rCTB的情况下，PPD刺激的脾细胞的肿瘤坏死因子（TNF）-α和干扰素（IFN）-γ产生以及抗原特异性脾细胞增殖均增强。这些结果强烈表明，rCTB增强细胞以及体液免疫应答。
Recombinant cholera toxin B subunit (rCTB) is a safe and potent mucosal adjuvant. To gain insight into the mechanism underlying the adjuvant effect of rCTB, the effects of rCTB on cell‐mediated immune responses of mice and guinea pigs were examined after intranasal administration of Mycobacterium bovis‐bacillus Calmette‐Guérin (BCG) with and without rCTB. Delayed‐type hypersensitivity, for skin reactions in guinea pigs and for footpad swelling reactions in mice, to purified protein derivative (PPD) were enhanced by intranasal co‐administration of BCG and rCTB, as compared to giving BCG alone to these animals. Moreover, tumor necrosis factor (TNF)‐α and interferon (IFN)‐γ production of spleen cells and antigen specific spleen cell proliferation, stimulated with PPD, were enhanced in the presence of rCTB. These results strongly suggest that rCTB enhances cellular as well as humoral immune responses.
霍乱毒素粘膜刺激后小鼠的全身和粘膜免疫。
DOI：
--
发表时间：
1984
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Elson,CO;Ealding,W
通讯作者：
Ealding,W