Effect of ultrasound on DNA polymerase reactions: Monitoring on a 27-MHz quartz crystal microbalance
Effect of ultrasound on DNA polymerase reactions: Monitoring on a 27-MHz quartz crystal microbalance
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DOI：
10.1021/bm050738e
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发表时间：
2006-03-01
影响因子：
6.2
通讯作者：
Okahata, Y
中科院分区：
文献类型：
--
作者：
Hoshino, Y;Kawasaki, T;Okahata, Y
在模板/引物DNA固定石英晶体微天平（QCM）上研究了超声波照射对DNA聚合酶（Klenow片段，KF）反应的影响。在超声波照射下，由于结合速率常数(k(1))的降低和解离速率常数(k(-1))的增加，KF与DNA的结合受到抑制。超声波照射提高了催化伸长率(k(cat))，但KF/DNA/单体三元复合物(K-m)的稳定性降低。超声效应与 KF 中域结构构象变化的相关性进行了讨论。
Effects of ultrasound irradiation on DNA polymerase (Klenow fragment, KF) reactions were studied on the template/ primer DNA-immobilized quartz crystal microbalance (QCM). Under ultrasound irradiation, binding of KF to the DNA was suppressed due to the decrease of the binding rate constant (k(1)) and the increase of the dissociation rate constant (k(-1)). The catalytic elongation rate (k(cat)) was increased, but the stability of the KF/DNA/monomer ternary complex (K-m) was decreased by the ultrasound irradiation. Ultrasound effects are discussed in correlation with the conformation changes of domain structures in KF.