Immunologic components in human milk during the second year of lactation.
Immunologic components in human milk during the second year of lactation.
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哺乳期第二年母乳中的免疫成分。
DOI：
10.1111/j.1651-2227.1983.tb09748.x
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Garza,C
中科院分区：
文献类型：
--
作者：
Goldman,AS;Goldblum,RM;Garza,C
在一项横断面研究中，5名妇女分别在12、13至15或16至24个月的哺乳期捐献母乳。每个妇女用低压电泵抽干乳房中的乳汁，收集一个样本。台盼蓝排除法鉴定活白细胞。电免疫扩散法测定乳铁蛋白和溶菌酶；荧光免疫吸附法测定总免疫球蛋白A和分泌免疫球蛋白A。用酶联免疫吸附法对大肠杆菌抗原的分泌IgA抗体进行了估计。标本中未见白细胞。总IgA和分泌IgA、乳铁蛋白和溶菌酶的浓度与最后6个月不间断哺乳和哺乳6 ~ 9个月逐渐断奶时的浓度相似。研究结果还表明，IgA抗体会导致脚趾。大肠杆菌是在哺乳期第二年产生的。这表明肠乳淋巴细胞运输通路（Peyer’s patches→肠系膜淋巴结→胸导管→血液→乳腺）在整个哺乳期都在运作，导致乳腺淋巴样细胞的发育，产生针对肠道微生物的IgA抗体。
In a cross-sectional study, 5 women donated milk for 12, 13 to 15 or 16 to 24 months of lactation. One specimen was collected from each woman by emptying a breast of milk with a low pressure electric pump. Viable leucocytes were identified by trypan blue exclusion. Lactoferrin and lysozyme were estimated by electroimmunodiffusion; total and secretory immunoglobulin (Ig)A were estimated by fluorescent immunosorbence. Secretory IgA antibodies to a pool ofEscherichia colisomatic antigens were estimated by enzyme-linked immunosorbence. No leucocyte was detected in any specimen. The concentrations of total and secretory IgA, lactoferrin and lysozyme were similar to those found in the last 6 months of uninterrupted lactation and during gradual weaning at 6 to 9 months of lactation. The findings also indicate that IgA antibodies toE. coliare produced during the second year of lactation. That suggests that the enteromammary lymphocyte traffic pathway (Peyer's patches→mesenteric lymph nodes→thoracid duct→blood→mammary gland), which leads to the development of lymphoid cells in the mammary gland which produce IgA antibodies to enteric microorganisms, operates throughout lactation.