Ultrasonic and spectroscopic studies on photoactivation of euglena
Ultrasonic and spectroscopic studies on photoactivation of euglena
复制标题
眼虫光活化的超声和光谱研究
DOI：
10.1063/1.2388736
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发表时间：
2006
影响因子：
3.2
通讯作者：
S. Morita
中科院分区：
文献类型：
--
作者：
M. Saito;S. Morita
研究了不同波长的紫外线照射对光合裸藻活性的影响。超声波操作技术被用于在光谱测量中的活动评估和运动限制。在黑暗条件下保存的裸藻细胞变得不活跃，因此大多数裸藻细胞被超声驻波（0.8mW scinmm ~ 2）捕获。然而，当它们暴露于500或700 nm波长的光（0.13W闪烁2）时，它们变得足够活跃，可以逃脱陷阱。相反，550、600或650 nm波长的照射对它们的活性没有影响。通过在超声驻波的一个节点处捕获裸藻，成功地进行了光谱测量，这对于运动微生物来说是困难的。在500或700 nm附近观察到吸收带，这对应于激活裸藻的照射波长。
We studied the effect of the irradiation wavelength on the activity of photosynthetic euglena. The ultrasonic manipulation technique was used for both the activity evaluation and the movement restriction in the spectral measurements. Euglenas that had been preserved in darkness became inactive, and accordingly most of them were trapped by the ultrasonic standing wave (0.8mW∕mm2). However, when they were exposed to light of 500 or 700nm wavelength (0.13W∕m2), they became active enough to escape from the trapping. By contrast, irradiation at 550, 600, or 650nm wavelength had no effect on their activity. Spectroscopic measurements, which used to be difficult for locomotive microorganisms, were conducted successfully by trapping euglena at a node of the ultrasonic standing wave. The absorption bands were observed at around 500 or 700nm, which corresponded to the irradiation wavelengths that activated euglena.