Host Responses to Persistent Mycobacterium avium Subspecies paratuberculosis Infection in Surgically Isolated Bovine Ileal Segments
Host Responses to Persistent Mycobacterium avium Subspecies paratuberculosis Infection in Surgically Isolated Bovine Ileal Segments
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DOI：
10.1128/cvi.00496-12
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发表时间：
2013-02-01
影响因子：
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通讯作者：
Griebel, Philip J.
中科院分区：
文献类型：
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作者：
Charavaryamath, Chandrashekhar;Gonzalez-Cano, Patricia;Griebel, Philip J.
缺乏适当的疾病模型限制了我们对鸟分枝杆菌亚种持续性肠道感染发病机制的理解。副结核。开发了一种模型，用于控制特定剂量的鸟分枝杆菌亚种的递送。手术分离新生小牛回肠节的副结核。稳定的肠段可以表征宿主对持久性鸟分枝杆菌亚种的反应。副结核菌感染9个月后，包括相对于相邻未感染肠室的局部粘膜免疫反应分析。鸟分枝杆菌亚种副结核仍然局限于肠道感染的初始部位，在肠系膜淋巴结中未被PCR检测到。鸟分枝杆菌亚种副结核特异性T细胞增殖反应包括引流肠系膜淋巴结的CD4和γ δ T细胞受体（γ δ TcR） T细胞反应。CD8（+）和γ δ TcR+ T细胞水平显著升高(P
A lack of appropriate disease models has limited our understanding of the pathogenesis of persistent enteric infections with Mycobacterium avium subsp. paratuberculosis. A model was developed for the controlled delivery of a defined dose of M. avium subsp. paratuberculosis to surgically isolated ileal segments in newborn calves. The stable intestinal segments enabled the characterization of host responses to persistent M. avium subsp. paratuberculosis infections after a 9-month period, including an analysis of local mucosal immune responses relative to an adjacent uninfected intestinal compartment. M. avium subsp. paratuberculosis remained localized at the initial site of intestinal infection and was not detected by PCR in the mesenteric lymph node. M. avium subsp. paratuberculosis-specific T cell proliferative responses included both CD4 and gamma delta T cell receptor (gamma delta TcR) T cell responses in the draining mesenteric lymph node. The levels of CD8(+) and gamma delta TcR+ T cells increased significantly (P