Rapid and serum-insensitive endocytotic delivery of proteins using biotinylated polymers attached via multivalent hydrophobic anchors.
Rapid and serum-insensitive endocytotic delivery of proteins using biotinylated polymers attached via multivalent hydrophobic anchors.
复制标题
DOI：
10.1016/j.jconrel.2013.12.024
复制
发表时间：
2014-03
期刊：
影响因子：
--
通讯作者：
Kyohei Tobinaga;Cuicui Li;Masafumi Takeo;Masayoshi Matsuda;Hiroko Nagai;T. Niidome;Tatsuhiro Yamamoto-Tatsuhiro-Y
中科院分区：
文献类型：
--
作者：
Kyohei Tobinaga;Cuicui Li;Masafumi Takeo;Masayoshi Matsuda;Hiroko Nagai;T. Niidome;Tatsuhiro Yamamoto-Tatsuhiro-Y
我们设计了生物素化聚合物作为具有多个烷基的合成受体，用于靶蛋白的内吞递送。聚合物通过多价锚定稳定地附着在细胞表面。该生物素在细胞表面与链亲和素（SA）结合，并通过内吞途径，细胞迅速内化生物素化聚合物/SA复合物。10%胎牛血清不抑制细胞对复合物的摄取，与商业试剂和单锚定型合成受体相比，其对SA的摄取效率最高。利用SA作为靶蛋白与细胞表面呈现的生物素之间的衔接分子，合成受体介导的内吞作用可广泛用于其他蛋白质。
We have designed biotinylated polymers as synthetic receptors that have multiple alkyl groups for endocytotic delivery of target proteins. The polymers were stably attached to a cell surface via multivalent anchoring. The presented biotin was bound to streptavidin (SA) on the cell surface, and, via an endocytotic pathway, the cell rapidly internalized the biotinylated polymer/SA complex. The cell's uptake of the complex was not inhibited by the presence of 10% fetal bovine serum, and its efficacy for the uptake of SA was the highest when compared with commercial reagents and single-anchored-type synthetic receptors. The synthetic receptor-mediated endocytosis can be used generally for other kind of protein by using SA as an adaptor molecule between a target protein and the cell-surface presented biotin.