Synaptic pruning through glial synapse engulfment upon motor learning
Synaptic pruning through glial synapse engulfment upon motor learning
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运动学习时通过神经胶质突触吞噬进行突触修剪
DOI：
10.1038/s41593-022-01184-5
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发表时间：
2022
影响因子：
25
通讯作者：
Fukazawa
中科院分区：
文献类型：
--
作者：
Morizawa Yosuke M.;Matsumoto Mami;Nakashima Yuka;Endo Narumi;Aida Tomomi;Ishikane Hiroshi;Beppu Kaoru;Moritoh Satoru;Inada Hitoshi;Osumi Noriko;Shigetomi Eiji;Koizumi Schuichi;Yang Guang;Hirai Hirokazu;Tanaka Kohichi;Tanaka Kenji F.;Ohno Nobuhiko;Fukazawa
突触修剪是学习和记忆中神经回路优化的基本过程。越来越多的证据表明，胶质细胞通过突触吞噬参与塑造神经元回路。然而，神经胶质参与突触修剪是否在记忆形成中起作用仍然是难以捉摸的。使用新开发的吞噬报告小鼠和三维超微结构表征，我们发现，小脑Bergmann胶质细胞（BG）的突触吞噬经常发生在小脑依赖的运动学习小鼠。我们观察到，随着学习后脊髓体积的减少，BG沿着突触前和突触后的蚕食增加。用膜联蛋白V药理学阻断吞噬抑制了脊柱体积减小和运动适应的过夜改善。这些结果表明，BG有助于完善成熟的小脑皮层电路通过突触吞噬在运动学习。
Synaptic pruning is a fundamental process of neuronal circuit refinement in learning and memory. Accumulating evidence suggests that glia participates in sculpting the neuronal circuits through synapse engulfment. However, whether glial involvement in synaptic pruning has a role in memory formation remains elusive. Using newly developed phagocytosis reporter mice and three-dimensional ultrastructural characterization, we found that synaptic engulfment by cerebellar Bergmann glia (BG) frequently occurred upon cerebellum-dependent motor learning in mice. We observed increases in pre- and postsynaptic nibbling by BG along with a reduction in spine volume after learning. Pharmacological blockade of engulfment with Annexin V inhibited both the spine volume reduction and overnight improvement of motor adaptation. These results indicate that BG contribute to the refinement of the mature cerebellar cortical circuit through synaptic engulfment during motor learning.