The period gene and allochronic reproductive isolation in Bactrocera cucurbitae
The period gene and allochronic reproductive isolation in Bactrocera cucurbitae
复制标题
DOI：
10.1098/rspb.2002.2152
复制
发表时间：
2002-12-07
影响因子：
4.7
通讯作者：
Tanimura, T
中科院分区：
文献类型：
--
作者：
Miyatake, T;Matsumoto, A;Tanimura, T
多效控制昼夜节律和交配时间的时钟基因可能导致瓜实蝇异时生殖隔离。具有较短的昼夜节律周期的苍蝇（约。22小时的自发活动节律）交配5小时前的一天比那些具有较长的昼夜节律周期（约。30 h）。择偶试验表明，短期和长期的昼夜节律的人群之间的显着交配前隔离。交配前的隔离没有发生时，交配时间同步的两个群体之间的光周期控制，表明生殖隔离是由于交配时间的变化，而不是任何不明行为学差异的两个群体之间。我们克隆了B的period（per）基因。与果蝇中的per基因同源的葫芦科植物。在光暗条件下，瓜蝇的每mRNA的相对水平表现出强大的每日波动。暗：暗条件下PER表达的波动与B的运动节律密切相关。葫芦科这些结果表明，时钟基因可以通过改变交配时间的多效性效应引起生殖隔离。
Clock genes that pleiotropically control circadian rhythm and the time of mating may cause allochronic reproductive isolation in the melon fly Bactrocera cucurbitae (Coquillett) (Diptera: Tephritidae). Flies with a shorter circadian period (ca. 22 h of locomotor activity rhythm) mated 5 h earlier in the day than those with a longer circadian period (ca. 30 h). Mate-choice tests demonstrated significant pre-mating isolation between populations with short and long circadian periods. Pre-mating isolation did not occur when the mating time was synchronized between the two populations by photoperiodic controls, indicating that reproductive isolation is due to variations in the time of mating and not any unidentified ethological difference between the two populations. We cloned the period (per) gene of B. cucurbitae that is homologous to the per gene in Drosophila. The relative level of per mRNA in the melon fly exhibited a robust daily fluctuation under light : dark conditions. The fluctuation of per expression under dark : dark conditions is closely correlated to the locomotor rhythm in B. cucurbitae. These results suggest that clock genes can cause reproductive isolation via the pleiotropic effect as a change of mating time.