The Calgary Biofilm Device: New technology for rapid determination of antibiotic susceptibilities of bacterial biofilms
The Calgary Biofilm Device: New technology for rapid determination of antibiotic susceptibilities of bacterial biofilms
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DOI：
10.1128/jcm.37.6.1771-1776.1999
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发表时间：
1999-06-01
影响因子：
9.4
通讯作者：
Buret, A
中科院分区：
文献类型：
--
作者：
Ceri, H;Olson, ME;Buret, A
基于抗生素对嗜酸菌的活性来测定抗生素敏感性是抗生素敏感性测试的标准测定。粘附的细菌群体（生物膜）存在先天缺乏抗生素敏感性，这在作为抗生素敏感性群体生长的相同细菌中未见，卡尔加里生物膜装置（CBD）被描述为用于快速和可再现地测定生物膜对抗生素敏感性的新技术。CBD产生96个等效生物膜，用于通过标准96孔技术测定抗生素亲和性。通过定量微生物学和扫描电子显微镜观察生物膜形成，确定美国临床实验室标准委员会（NCCLS）参考菌株对标准抗生素组的敏感性：大肠杆菌ATCC 25922、铜绿假单胞菌ATCC 27853和金黄色葡萄球菌ATCC 29213，生长曲线表明，在特定的时间点，可以在CBD上形成预定尺寸的生物膜，此外，在96个钉中的每一个上形成的生物膜之间没有观察到显著差异（P > 0.1）。通过NCCLS方法或从CBD获得的抗生素敏感性的抗生素敏感性是相似的。通过使用CBD获得的最小生物膜根除浓度表明，对于相同生物体的生物膜，通常需要100至1，000倍浓度的某种抗生素才能有效，而其他抗生素在MIC下有效，CBD为合理选择对微生物生物膜有效的抗生素和筛选新的有效抗生素化合物提供了新技术。
Determination of the MIG, based on the activities of antibiotics against planktonic bacteria, is the standard assay for antibiotic susceptibility testing. Adherent bacterial populations (biofilms) present with an innate lack of antibiotic susceptibility not seen in the same bacteria grown as planktonic populations, The Calgary Biofilm Device (CBD) is described as a new technology for the rapid and reproducible assay of biofilm susceptibilities to antibiotics. The CBD produces 96 equivalent biofilms for the assay of antibiotic susceptibilities by the standard 96-well technology. Biofilm formation was followed by quantitative microbiology and scanning electron microscopy, Susceptibility to a standard group of antibiotics was determined for National Committee for Clinical Laboratory Standards (NCCLS) reference strains: Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, and Staphylococcus aureus ATCC 29213, Growth curves demonstrated that biofilms of a predetermined size could be formed on the CBD at specific time points and, furthermore, that no significant difference (P > 0.1) was seen between biofilms formed on each of the 96 pegs. The antibiotic susceptibilities for planktonic populations obtained by the NCCLS method or from the CBD were similar. Minimal biofilm eradication concentrations, derived by using the CBD, demonstrated that for biofilms of the same organisms, 100 to 1,000 times the concentration of a certain antibiotic were often required for the antibiotic to be effective, while other antibiotics were found to be effective at the MICs, The CBD offers a new technology for the rational selection of antibiotics effective against microbial biofilms and for the screening of new effective antibiotic compounds.