Secretion of human β-interferon into the cystic fluid of glioma transfected with the interferon gene
Secretion of human β-interferon into the cystic fluid of glioma transfected with the interferon gene
复制标题
转染干扰素基因的神经胶质瘤囊液中分泌人β-干扰素
DOI：
10.3164/jcbn.11.123
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发表时间：
1991
影响因子：
2.4
通讯作者：
K. Yagi
中科院分区：
文献类型：
--
作者：
J. Yoshida;M. Mizuno;K. Yagi
以人胶质瘤细胞U251-SP产生的皮下实体瘤为研究对象，采用新型脂质体转染人β-干扰素（HuIFN-β）基因。当肿瘤直径超过15mm时，通常形成单个或多个囊肿，我们将包裹基因的脂质体注射到囊肿中，发现HuIFN-β分泌到囊液中。转染48h后达到450IU/ml左右，并维持8天。每隔一天注射一次，连续10天，肿瘤的生长完全被抑制。肿瘤在30天内根本没有生长。
Using subcutaneous solid tumor produced by U251-SP human glioma cells, we studied the in vivo transfection of the cells with the human β-interferon (HuIFN-β) gene by means of novel liposomes. Since the tumor usually forms a single or multiple cysts when the tumor becomes over 15mm in diameter, we injected liposomes entrapping the gene into the cyst, and found that HuIFN-β was secreted into the cystic fluid. It reached a level of approximately 450IU/ml 48h after the transfection and remained at the same level for 8 days. The growth of the tumor was completely inhibited when the injection was carried out every second day for 10 days. The tumor did not grow at all for 30 days.