Biological monitoring of pyrethroids in blood and pyrethroid metabolites in urine: Applications and limitations
Biological monitoring of pyrethroids in blood and pyrethroid metabolites in urine: Applications and limitations
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DOI：
10.1016/s0048-9697(97)05493-4
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发表时间：
1997-06-20
影响因子：
9.8
通讯作者：
Idel, H
中科院分区：
文献类型：
--
作者：
Leng, G;Kuhn, KH;Idel, H
本研究的目的是对职业接触拟除虫菊酯的受试者进行生物学监测。研究小组由30名接触过氟氯菊酯、氯氰菊酯或氯菊酯的虫害防治工作人员组成。暴露后采集24 h尿样并抽血20 ml。采用气相色谱质谱法测定尿液中拟除虫菊酯代谢物顺式和反式3-（2,2-二氯乙烯基）-2,2-二甲基环丙甲酸、3-苯氧苯甲酸和氟苯氧苯甲酸（检出限为0.5 μ g/l），采用气相色谱- ecd法测定血浆中拟除虫菊酯（检出限为5 μ g/l）。除虫人员尿液中代谢物浓度在< 0.5 μ g/l至277 μ g/l之间。血浆中氟氯菊酯、氯氰菊酯和氯菊酯浓度均低于检测限（< 5 μ g/l）。为了测试代谢物是否对拟除虫菊酯暴露有特异性，在未暴露受试者的尿液中测定了代谢物（n = 40）。所有病例均未检出拟除虫菊酯代谢物。氟氯菊酯消除实验表明，氟氯菊酯代谢物的消除遵循一级动力学（t(1/2) = 6.4 h）。储存实验表明，冷冻尿液样本（-21℃）在一年内没有明显的代谢物损失。相反，储存在血浆中的拟除虫菊酯容易进一步发生生物变性。(C) 1997爱思唯尔科学有限公司
The objective of this study was to perform biological monitoring of subjects who are occupationally exposed to pyrethroids. The study group consisted of 30 pest control operators exposed to cyfluthrin, cypermethrin or permethrin. After exposure, 24-h urine samples were collected and 20 ml of blood was drawn. The pyrethroid metabolites cis- and trans-3-(2,2-dichlorovinyl)-2,2-dimethylcyclopropanecarboxylic acid, 3-phenoxybenzoic acid and fluorophenoxybenzoic acid were determined in the urine samples (limit of detection: 0.5 mu g/l) by GC MS and the pyrethroids in plasma (limit of detection: 5 mu g/l) by GC-ECD. The concentrations of metabolites in the urine of the pest control operators ranged between < 0.5 mu g/l and 277 mu g/l urine. The concentrations of cyfluthrin, cypermethrin and permethrin in the plasma were below the limits of detection (< 5 mu g/l). To test if the metabolites are specific for pyrethroid exposure, they were determined in the urine of non-exposed subjects (n = 40). In no case could pyrethroid metabolites be detected. A cyfluthrin elimination experiment showed that cyfluthrin metabolites are eliminated following first-order kinetics (t(1/2) = 6.4 h). Storage experiments demonstrate that frozen urine samples (-21 degrees C) show no significant losses of metabolites within a year. In contrast, pyrethroids stored in plasma are susceptible to further biodegeneration. (C) 1997 Elsevier Science B.V.