Engineering discoidal polymeric nanoconstructs with enhanced magneto-optical properties for tumor imaging
Engineering discoidal polymeric nanoconstructs with enhanced magneto-optical properties for tumor imaging
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DOI：
10.1016/j.biomaterials.2013.03.078
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发表时间：
2013-07-01
期刊：
影响因子：
14
通讯作者：
Decuzzi, Paolo
中科院分区：
文献类型：
--
作者：
Key, Jaehong;Aryal, Santosh;Decuzzi, Paolo
纳米粒子的体内性能受到其尺寸、形状和表面性质的影响。基于乳化和纳米沉淀的制造方法无法在多个尺度上精确且独立地控制这些特征。在此，通过改进的水凝胶模板策略展示了直径为 1000 nm、高度为 500 am 的盘状聚合物纳米结构（DPN）。 DPNs 是通过在一个合成步骤中混合组成聚合物 - 聚乳酸-乙醇酸 (PLGA) 和聚乙二醇 (PEG) 氯甲基丙烯酸酯 - 以及具有磁光特性的有效负载 -5 am 超小超顺磁性氧化铁纳米颗粒 (SPIO) 和罗丹明 B 染料 (RhB) 来获得的。 DPN 几何特征通过多种显微镜技术来表征。罗丹明 B 染料的释放取决于 pH 值，并且在酸性条件下通过增强聚合物基质的水解而增加。每个 DPN 装载有大约 100 fg 的铁，可以被静态磁场和外部磁场有效地拖动。此外，DPN 多孔结构内的 USPIO 限制导致 MRI 弛豫率显着增强（r(2) 接近 500 (mMs)(-1)），比市售系统高出 5 倍。这些纳米结构提出了设计用于抗血管生成治疗和血管成像的治疗诊断系统的一般策略。 (C) 2013 Elsevier Ltd. 保留所有权利。
The in vivo performance of nanoparticles is affected by their size, shape and surface properties. Fabrication methods based on emulsification and nano-precipitation cannot control these features precisely and independently over multiple scales. Herein, discoidal polymeric nanoconstructs (DPNs) with a diameter of 1000 nm and a height of 500 am are demonstrated via a modified hydrogel-template strategy. The DPNs are obtained by mixing in one synthesis step the constituent polymers - poly(lactic acid-co-glycolic acid) (PLGA) and polyethylene glycol (PEG) climethacrylate - and the payload with magneto-optical properties -5 am ultra-small super-paramagnetic iron oxide nanoparticles (SPIOs) and Rhodamine B dye (RhB). The DPN geometrical features are characterized by multiple microscopy techniques. The release of the Rhodamine B dye is pH dependent and increases under acidic conditions by the enhanced hydrolysis of the polymeric matrix. Each DPN is loaded with similar to 100 fg of iron and can be efficiently dragged by static and external magnetic fields. Moreover, the USPIO confinement within the DPN porous structure is responsible for a significant enhancement in MRI relaxivity (r(2) similar to 500 (mMs)(-1)), up to similar to 5 times larger than commercially available systems. These nanoconstructs suggest a general strategy to engineer theranostic systems for anti-angiogenic treatment and vascular imaging. (C) 2013 Elsevier Ltd. All rights reserved.