Multi-residue Analysis of Residual Veterinary Drugs in Muscle Using Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry
Multi-residue Analysis of Residual Veterinary Drugs in Muscle Using Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry
复制标题
液相色谱-电喷雾电离串联质谱法对肌肉中兽药残留的多残留分析
DOI：
10.11468/seikatsueisei.49.152
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
M. Kitayama
中科院分区：
文献类型：
--
作者：
Ryoji Yamada;Masaki Kozono;T. Ohmori;M. Kato;F. Morimatsu;M. Kitayama
建立了一种快速筛选肌肉中兽药残留的LC/MS/MS方法。采用乙腈-甲醇（95:5,v/v）法从牛背最长肌（L. dorsi）、猪背最长肌（L. dorsi）和鸡胸大肌中提取药物（3种抗生素、18种合成抗菌药物、10种驱虫剂和3种合成代谢剂），用乙腈饱和正己烷冲洗后蒸发。用甲醇溶解，C18柱梯度洗脱液相色谱分析，电喷雾电离串联质谱法检测。检出限为0.03 ~ 2.0 ng/g。33种药物的回收率在71~ 119%之间。
A simple and rapid screening method using LC/MS/MS for the multi-residue analysis of residual veterinary drugs in muscle was developed. The drugs (3 antibiotics, 18 synthetic antibacterials, 10 anthelminthics and 3 anabolic agents) were extracted from bovine longissimus dorsi (L. dorsi), porcine L. dorsi and chicken Pectoralis major with acetonitrile-methanol (95:5, v/v), and the extracts were rinsed with n-hexane saturated with acetonitrile and evaporated. The residues were dissolved with methanol and analyzed by liquid chromatography with gradient elution on a C18 column and detected by electrospray ionization tandem mass spectrometry. The detection limits were 0.03 to 2.0 ng/g. The recovery rates of the 33 drugs were in the range of 71~119 %.