PHOTO-CIDNP OF THE AMINO-ACIDS
PHOTO-CIDNP OF THE AMINO-ACIDS
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DOI：
10.1111/j.1751-1097.1989.tb08425.x
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发表时间：
1989-05-01
影响因子：
3.3
通讯作者：
KAPTEIN, R
中科院分区：
文献类型：
--
作者：
STOB, S;KAPTEIN, R
一个光化学诱导的动态核极化（光CIDNP）的研究提出的氨基酸，是可极化的黄素染料。这些包括色氨酸、酪氨酸、组氨酸、甲基化赖氨酸和甲硫氨酸的衍生物。的pH值，浓度和化学改性的CIDNP效应的大小的影响进行了研究，以获得有关的自由基对形成的机械信息。酪氨酸和色氨酸极化的pH和浓度依赖性可以定量地解释。CIDNP的证据表明，氢原子的提取是重要的，在产生自由基对的情况下，组氨酸和酪氨酸，而电子转移盛行的情况下，色氨酸，甲基化的赖氨酸和蛋氨酸。
A photochemically induced dynamic nuclear polarisation (photo-CIDNP) study is presented of the amino acids that are polarisable with a flavin dye. These include derivatives of tryptophan, tyrosine, histidine, methylated lysines and methionine. The influence of pH, concentration and chemical modification on the magnitude of the CIDNP effect has been studied to obtain mechanistic information about the radical pair formation. The pH and concentration dependence of tyrosine and tryptophan polarisation could be accounted for quantitatively. The CIDNP evidence indicates that hydrogen-atom abstraction is important in generating radical pairs in the case of histidine and tyrosine, while electron transfer prevails in the case of tryptophan, the methylated lysines and methionine.