ISOLATION AND ANALYSIS OF EMBRYONIC EXPRESSION OF HOX-4.9, A MEMBER OF THE MURINE LABIAL-LIKE GENE FAMILY
ISOLATION AND ANALYSIS OF EMBRYONIC EXPRESSION OF HOX-4.9, A MEMBER OF THE MURINE LABIAL-LIKE GENE FAMILY
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DOI：
10.1016/0925-4773(92)90038-l
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发表时间：
1992-07-01
影响因子：
2.6
通讯作者：
MARTIN, GR
中科院分区：
文献类型：
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作者：
FROHMAN, MA;MARTIN, GR
小鼠唇（实验室）亚家族的两个成员，Hox-1.6和Hox-2.9，含有类果蝇同源框基因，以前已经确定。在这里，我们描述了第三个成员遗传连锁的Hox-4簇2号染色体上。这个基因被命名为Hox-4.9，在结构上与其他实验室亚家族成员相似。然而，除了同源框和紧邻其上游的序列之外，几乎没有编码序列是保守的。通过原位杂交分析，Hox-4.9 mRNA首次检测到的后期条纹阶段（E7.75）在推测的侧和胚外中胚层结束。在早期神经发生（E8.0-8.5），Hox-4.9仅在侧中胚层中检测到;其在体节中胚层和神经管中缺乏表达，使其在Hox基因中独一无二。到神经发生晚期和妊娠中期（E9.0-E11.5），Hox-4.9不再在外侧中胚层中检测到，而是在假定的躯干神经嵴的限制区域和皮节中发现。这些数据进行了讨论，与什么是已知的实验室亚家族的其他成员的表达进行比较。
Two members of the murine labial (lab) subfamily of Antennapedia-like homeobox-containing genes, Hox-1.6 and Hox-2.9, have been identified previously. Here we describe a third member genetically linked to the Hox-4 cluster on chromosome 2. This gene, designated Hox-4.9, is similar in structure to the other lab subfamily members. However, little coding sequence other than the homeobox and sequences immediately upstream of it have been conserved. By in situ hybridization analysis, Hox-4.9 mRNA is first detected at the end of the late streak stage (E7.75) in presumptive lateral and extraembryonic mesoderm. During early neurogenesis (E8.0-8.5), Hox-4.9 is detected solely in lateral mesoderm; its lack of expression in somitic mesoderm and the neural tube makes it unique among the Hox genes. By late neurogenesis and through mid-gestation (E9.0-E11.5), Hox-4.9 is no longer detected in lateral mesoderm but is found instead in a restricted region of presumed trunk neural crest and in the dermatome. These data are discussed in comparison with what is known about expression of the other members of the lab subfamily.