Movement and guidance of migrating mesodermal cells in Ambystoma maculatum gastrulae.
Movement and guidance of migrating mesodermal cells in Ambystoma maculatum gastrulae.
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发表时间：
1982-08
影响因子：
4
通讯作者：
N. Nakatsuji;A. Gould;K. Johnson
中科院分区：
文献类型：
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作者：
N. Nakatsuji;A. Gould;K. Johnson
扫描电子显微镜研究早期原肠胚的Ambystoma maculatum表明，迁移假定中胚层细胞强烈地朝向动物极。它们的前缘有片状伪足和丝状伪足，后缘呈圆形或锥形，尾状。的细胞，其极化可以明确地确定，81%似乎是针对在一个象限朝向动物极，和93%的定向在一定程度上远离胚孔。这种强烈的取向表明，除了细胞运动的接触抑制的非特异性分散机制之外，特异性机制指导细胞运动。外胚层的内表面覆盖着一层细小的细胞外纤维网。迁移细胞的丝状伪足经常附着在原纤维上，并似乎跟随原纤维，这表明原纤维作为细胞附着和运动的指导基质。存在纤维明显沿胚孔-动物极轴沿着排列的区域，并且使用高倍显微照片进行的初步统计分析显示与胚孔-动物极轴平行的显著排列。这种原纤维排列可以通过接触引导引起中胚层细胞的强烈取向。
A scanning electron microscopic study in early gastrulae of Ambystoma maculatum showed that migrating presumptive mesodermal cells were strongly oriented toward the animal pole. They had lamellipodia and filopodia at their leading edges, and rounded or tapering, tail-like, trailing edges. Of the cells whose polarization could be determined unequivocally, 81% appeared to be directed in a quadrant toward the animal pole, and 93% were directed to some extent away from the blastopore. This strong orientation suggests that specific mechanisms direct cell movement, in addition to the non-specific dispersive mechanism of the contact inhibition of cell movement. There is a network of fine extracellular fibrils that covers the inner surface of the ectodermal layer. Filopodia of the migrating cells frequently attach to and appear to follow the fibrils, suggesting that the fibrils serve as a guiding substratum for cell attachment and movement. There are areas where the fibrils are apparently aligned along the blastopore-animal pole axis, and a preliminary statistical analysis using micrographs at high magnification showed a significant alignment parallel to the blastopore-animal pole axis. This fibril alignment could cause the strong orientation of the mesodermal cells by means of contact guidance.