Identification and Dissection of Diverse Mouse Adipose Depots
Identification and Dissection of Diverse Mouse Adipose Depots
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DOI：
10.3791/59499
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发表时间：
2019-07-01
影响因子：
1.2
通讯作者：
MacDougald, Ormond A.
中科院分区：
文献类型：
--
作者：
Bagchi, Devika P.;MacDougald, Ormond A.
脂肪组织是具有广泛功能的复杂器官，包括响应局部和全局需求的能量储存和动员，代谢解偶联以产生热量，以及分泌脂肪因子以调节全身稳态和免疫反应。新兴的研究正在确定重要的区域差异，在发育，分子和功能概况的脂肪细胞位于离散仓库在整个身体。不同性质的仓库是医学相关的，因为代谢疾病往往表现出仓库的具体影响。该方案将为研究者提供详细的解剖图谱和解剖指南，用于可重复和准确地识别和切除各种小鼠脂肪组织。离散脂肪库的标准化解剖将允许详细比较其分子和代谢特征以及在各种营养和环境条件下对局部和全身病理状态的贡献。
Adipose tissues are complex organs with a wide array of functions, including storage and mobilization of energy in response to local and global needs, uncoupling of metabolism to generate heat, and secretion of adipokines to regulate whole-body homeostasis and immune responses. Emerging research is identifying important regional differences in the developmental, molecular, and functional profiles of adipocytes located in discrete depots throughout the body. Different properties of the depots are medically relevant since metabolic diseases often demonstrate depot-specific effects. This protocol will provide investigators with a detailed anatomic atlas and dissection guide for the reproducible and accurate identification and excision of diverse mouse adipose tissues. Standardized dissection of discrete adipose depots will allow detailed comparisons of their molecular and metabolic characteristics and contributions to local and systemic pathologic states under various nutritional and environmental conditions.