Exosomal microRNA miR-92a concentration in serum reflects human brown fat activity.
Exosomal microRNA miR-92a concentration in serum reflects human brown fat activity.
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DOI：
10.1038/ncomms11420
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发表时间：
2016-04-27
影响因子：
16.6
通讯作者：
Pfeifer A
中科院分区：
文献类型：
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作者：
Chen Y;Buyel JJ;Hanssen MJ;Siegel F;Pan R;Naumann J;Schell M;van der Lans A;Schlein C;Froehlich H;Heeren J;Virtanen KA;van Marken Lichtenbelt W;Pfeifer A
棕色脂肪组织（BAT）消耗能量，其活动与成年人的消瘦有关。18 F-氟脱氧葡萄糖（18 F-FDG）正电子发射断层扫描结合计算机断层扫描（PET/CT）仍然是测量BAT活性的标准，但会使受试者暴露于电离辐射。为了研究BAT在大型人群中的功能，需要新的诊断工具。在这里，我们表明棕色脂肪细胞释放外泌体，BAT激活增加外泌体释放。分析从棕色脂肪细胞释放的外泌体和从小鼠血清分离的外泌体中的miRNA，我们表明在体外和体内BAT激活后miRNA的水平发生变化。这些外泌体miRNA之一，miR-92 a，也存在于人血清外泌体中。重要的是，外泌体miR-92 a的血清浓度与通过18F-FDG PET/CT在包括41名健康个体的两个独特且独立的队列中测量的人BAT活性负相关。因此，外泌体miR-92 a代表了小鼠和人类中BAT活性的潜在血清生物标志物。 外来体是含有RNA的脂质囊泡，在组织间串扰中起作用。在本文中，作者表明，在体外和体内产热活化后，棕色脂肪细胞的外泌体释放增加，并证明外泌体miR-92的血清水平反映了人类的棕色脂肪活性。
Brown adipose tissue (BAT) dissipates energy and its activity correlates with leanness in human adults. 18F-fluorodeoxyglucose (18F-FDG) positron emission tomography coupled with computer tomography (PET/CT) is still the standard for measuring BAT activity, but exposes subjects to ionizing radiation. To study BAT function in large human cohorts, novel diagnostic tools are needed. Here we show that brown adipocytes release exosomes and that BAT activation increases exosome release. Profiling miRNAs in exosomes released from brown adipocytes, and in exosomes isolated from mouse serum, we show that levels of miRNAs change after BAT activation in vitro and in vivo. One of these exosomal miRNAs, miR-92a, is also present in human serum exosomes. Importantly, serum concentrations of exosomal miR-92a inversely correlate with human BAT activity measured by 18F-FDG PET/CT in two unique and independent cohorts comprising 41 healthy individuals. Thus, exosomal miR-92a represents a potential serum biomarker for BAT activity in mice and humans. Exosomes are RNA-containing lipid vesicles with roles in inter-tissue crosstalk. Here the authors show that exosome release from brown adipocytes is increased upon thermogenic activation, both in vitro and in vivo, and demonstrate that serum levels of exosomal miR-92 reflect brown fat activity in humans.