β-adaptin:: Key molecule for microglial scavenger receptor function under oxidative stress
β-adaptin:: Key molecule for microglial scavenger receptor function under oxidative stress
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DOI：
10.1016/j.bbrc.2006.10.077
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发表时间：
2006-12-22
影响因子：
3.1
通讯作者：
Mas-Oliva, Jaime
中科院分区：
文献类型：
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作者：
Manzano-Leon, Natalia;Delgado-Coello, Blanca;Mas-Oliva, Jaime
清道夫受体通过受体介导的内吞作用内化化学修饰的低密度脂蛋白颗粒（ac-LDL）和其他配体。在这项调查中使用淀粉样蛋白-β作为天然配体的SR，我们研究了在配体诱导的氧化应激条件下，在小胶质细胞和巨噬细胞的内吞机制的组织中重要的几个衔接蛋白的蛋白质表达的变化。根据剂量和暴露于淀粉样蛋白-β的时间进行RAW（巨噬细胞）和EOC（小胶质细胞）细胞中β-适应蛋白、α-适应蛋白、SR-AI和SR-BI的差异表达实验。我们的研究结果表明，根据剂量，淀粉样蛋白β产生氧化应激状态，重要地影响β-适应素的可用性。在这些条件下，RT-PCR测定显示β-adaptin mRNA正常合成，这是β-adaptin的蛋白质翻译或蛋白质结构可能改变的原因。这些观察结果可能会对理解小胶质细胞在大脑中处理淀粉样蛋白β的机制产生影响。(c)2006年爱思唯尔公司All rights reserved.
Scavenger receptors internalize chemically modified low density lipoprotein particles (ac-LDL) and other ligands through the process of receptor-mediated endocytosis. During this investigation using amyloid-beta as a natural ligand for the SR, we studied under a ligand-induced oxidative stress condition, changes in protein expression of several adaptor proteins important in the organization of the endocytic machinery in microglia and macrophages. Differential expression experiments of beta-adaptin, alpha-adaptin, SR-AI, and SR-BI in RAW (macrophages) and EOC (microglia) cells were performed according to dosage and exposure time to amyloid-beta. Our results show that according to dosage, amyloid-beta produces an oxidative stress state that importantly affects the availability of beta-adaptin. Under these conditions, RT-PCR assays show that beta-adaptin mRNA is normally synthesized, reason why protein translation or protein structure of beta-adaptin might be altered. These observations might have impact in the understanding of the mechanisms microglia employ to process amyloid-beta in the brain. (c) 2006 Elsevier Inc. All rights reserved.