Tide-related Changes in mRNA Abundance of Aromatases and Estrogen Receptors in the Ovary and Brain of the Threespot Wrasse Halichoeres trimaculatus
Tide-related Changes in mRNA Abundance of Aromatases and Estrogen Receptors in the Ovary and Brain of the Threespot Wrasse Halichoeres trimaculatus
复制标题
DOI：
10.1007/s12601-018-0016-0
复制
发表时间：
2018-05
影响因子：
1.3
通讯作者：
D. Oh;Sung-Pyo Hur;Selma Bouchekioua;Y. Takeuchi;Shingo Udagawa;N. Aluru;Yong‐Ju Park;J. Park;Se-Jae Kim;T. W. Moon;M. Vijayan;A. Takemura
中科院分区：
文献类型：
--
作者：
D. Oh;Sung-Pyo Hur;Selma Bouchekioua;Y. Takeuchi;Shingo Udagawa;N. Aluru;Yong‐Ju Park;J. Park;Se-Jae Kim;T. W. Moon;M. Vijayan;A. Takemura
三点濑鱼（Halichoeres trimaculatus; Family Labridae）是印度太平洋常见的珊瑚礁物种。鉴于该物种每天在涨潮时产卵，我们假设与性腺发育有关的内分泌变化与潮汐周期同步。为了验证这一点，我们检测了该物种卵巢和大脑中两种细胞色素P450芳香化酶（cyp19aandcyp19b）和两种雌激素受体（erαanderβ）对潮汐变化的转录丰度。在4个潮汐点[低潮（LT）、涨潮（FT）、高潮（HT）和退潮（ET）]前后采集鱼时，在高潮（HT）和落潮（ET）前后，促性腺指数和卵母细胞直径分别升高。在HT周围观察到卵巢有排卵卵泡。实时定量聚合酶链反应显示，卵巢中cyp19aanderα mRNA丰度在ET和HT前后分别升高，而notβ mRNA丰度则升高。另一方面，在FT周围，前脑cyp19 mRNA水平显著升高。在FT周围，大脑和中脑的所有区域均观察到α - anderβmRNA丰度的增加。特定潮汐周期生殖关键基因mRNA丰度的变化，以及卵巢卵黄细胞的发育，支持了我们的假设，即内分泌变化与潮汐周期同步，在该物种的性腺发育中起作用。我们假设脑内睾丸激素转化为E2可能与濑鱼的产卵行为有关，因为濑鱼在HT周围与一个占据领地的雄性进行群体产卵。
The threespot wrasse (Halichoeres trimaculatus; Family Labridae) is a common coral reef species of the Indo-Pacific Ocean. Given that this species spawns daily at high tide (HT), we hypothesized that endocrine changes in relation to gonadal development are synchronized with the tidal cycle. To test this, we examined the transcript abundance of two cytochrome P450 aromatases (cyp19aandcyp19b) and two estrogen receptors (erαanderβ) in the ovary and brain of this species in response to tidal change. When fish were collected around four tidal points [low tide (LT), flood tide (FT), high tide (HT), and ebb tide (ET)], gonadosomatic index and oocyte diameter increased around HT and FT, respectively. Ovulatory follicles were observed in ovaries around HT. Real-time quantitative polymerase-chain reaction revealed that mRNA abundance ofcyp19aanderα, but noterβ, in the ovary increased around ET and HT, respectively. On the other hand, mRNA levels ofcyp19bin the forebrain were significantly higher around FT. Increases oferαanderβmRNA abundance around FT were observed in all areas of the brain and the midbrain, respectively. The changes in mRNA abundance of key genes involved in reproduction at specific tidal cycles, along with the development of the vitellogenic oocytes in the ovary, support our hypothesis that synchronization of endocrine changes to the tidal periodicity plays a role in the gonadal development of this species. We hypothesize that conversion of testosterone to E2 in the brain may be associated with the spawning behavior given that the wrasse exhibits group spawning with a territory-holding male around HT.