Identification of small secreted peptides (SSPs) in maize and expression analysis of partial SSP genes in reproductive tissues
Identification of small secreted peptides (SSPs) in maize and expression analysis of partial SSP genes in reproductive tissues
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玉米小分泌肽（SSP）的鉴定及生殖组织中部分SSP基因的表达分析
DOI：
10.1007/s00425-014-2123-1
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发表时间：
2014-10-01
期刊：
影响因子：
4.3
通讯作者：
Zhang, Xian Sheng
中科院分区：
文献类型：
--
作者：
Li, Ye Long;Dai, Xin Ren;Zhang, Xian Sheng
主要结论鉴定出玉米1491条小分泌肽，并根据肽序列特征对其进行分类。采用qRT-PCR检测生殖组织中SSP基因的部分表达。小分泌肽（ssp）是植物细胞间重要的通讯信使。大多数关于植物ssp的信息来自拟南芥和水稻，而对玉米等其他禾本科植物的ssp知之甚少。在这项研究中，我们从玉米基因组序列中鉴定出1491个SSP基因。这些推测的SSP基因分布在10条玉米染色体上。其中611种ssp根据保守结构域划分为198个超科，725种在c端具有4个或更多半胱氨酸的ssp与其他植物物种的同类具有相似的半胱氨酸排列。此外，还鉴定了需要翻译后修饰的ssp以及防御素样蛋白（DEFL）。进一步分析了110个SSP基因在雄花、花粉、蚕丝和子房等生殖组织中的表达水平。编码基底层抗真菌肽样蛋白、小被膜蛋白样蛋白、硫氧还蛋白样蛋白、γ -硫蛋白样蛋白和DEFL蛋白的大部分基因在子房和雄花中的表达水平高于在蚕丝和成熟花粉中的表达水平。快速碱化因子样基因仅在成熟子房和成熟花粉中高表达，花粉ole1样基因在蚕丝中低表达。本研究结果为进一步分析SSP在玉米生殖过程中的作用提供了基础资料。
Main conclusion Maize 1,491 small secreted peptides were identified, which were classified according to the character of peptide sequences. Partial SSP gene expressions in reproductive tissues were determined by qRT-PCR.Small secreted peptides (SSPs) are important cell-cell communication messengers in plants. Most information on plant SSPs come from Arabidopsis thaliana and Oryza sativa, while little is known about the SSPs of other grass species such as maize (Zea mays). In this study, we identified 1,491 SSP genes from maize genomic sequences. These putative SSP genes were distributed throughout the ten maize chromosomes. Among them, 611 SSPs were classified into 198 superfamilies according to their conserved domains, and 725 SSPs with four or more cysteines at their C-termini shared similar cysteine arrangements with their counterparts in other plant species. Moreover, the SSPs requiring post-translational modification, as well as defensin-like (DEFL) proteins, were identified. Further, the expression levels of 110 SSP genes were analyzed in reproductive tissues, including male flower, pollen, silk, and ovary. Most of the genes encoding basal-layer antifungal peptide-like, small coat proteins-like, thioredoxin-like proteins, gamma-thionins-like, and DEFL proteins showed high expression levels in the ovary and male flower compared with their levels in silk and mature pollen. The rapid alkalinization factor-like genes were highly expressed only in the mature ovary and mature pollen, and pollen Ole e 1-like genes showed low expression in silk. The results of this study provide basic information for further analysis of SSP functions in the reproductive process of maize.