Automatic Turgor Pressure Recording in Plant Cells
Automatic Turgor Pressure Recording in Plant Cells
复制标题
植物细胞中的自动膨胀压力记录
DOI：
10.1515/znc-1987-9-1025
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
U. Zimmermann
中科院分区：
文献类型：
--
作者：
K. Buchner;G. Wehner;W. Virsik;U. Zimmermann
本文介绍了一种自动调节压力探头，它便于直接和连续地测量植物细胞内的膨压和水分关系参数。光电传感器驱动反馈系统，使得测量毛细管中的油/细胞汁液界面的位置保持恒定。通过将传感器安装在与用于观察的显微镜相连的聚焦屏上，该传感器可轻松应用于大多数测量设置。油/细胞汁液边界的位置通过其沿着屏幕上毛细管图像的移动来感测，并且可以被设置到任何适当的位置。调节信号的光耦合是有利的，因为它使实验者自由地测量细胞的电参数或其他参数。对绿色假单胞菌细胞的实验表明，该装置可以更快，更准确地测量水的关系参数，并在更长的时间内，比以前的方法。
An automatically-regulated pressure probe is described, which facilitates the direct and continuous measurement of turgor pressure and water relation parameters in plant cells. An optoelectronic sensor drives a feed back system so that the position of the oil/cell sap interface in the measuring capillary remains constant. The sensor is easily applied to most measurement set-ups by mounting it on a focusing screen connected to the microscope used for observation. The position of the oil/cell sap boundary is sensed by its movement along the image of the capillary on the screen and can be set to any appropriate position. The optical coupling of the regulation signal is advantageous because it leaves the experimenter free to measure the electrical or other parameters of the cell. Experiments on cells of Eremosphaera viridis have shown that the device allows much faster and more exact measurements of water relation parameters, and over longer periods, than the earlier method.