Ionic-liquid-based catch and release mass spectroscopy tags for enzyme monitoring
Ionic-liquid-based catch and release mass spectroscopy tags for enzyme monitoring
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DOI：
10.1039/c0cc04224b
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发表时间：
2010-01-01
影响因子：
4.9
通讯作者：
Bernard, Claire
中科院分区：
文献类型：
--
作者：
Galan, M. Carmen;Tran, Anh Tuan;Bernard, Claire
一种新颖、廉价、通用的基于离子液体的捕获和释放质谱标签(I-TAG)已被开发出来，它有助于底物纯化、快速、可靠和灵敏的酶反应监测和定量动力学分析。该体系在β-1，4-半乳糖基转移酶的酶促测定中得到了验证。
A novel, inexpensive and versatile ionic-liquid-based catch and release mass spectrometry tag (I-Tag) that facilitates substrate purification, fast, robust and sensitive enzymatic reaction monitoring and quantitative kinetic analysis has been developed. The applicability of the system has been demonstrated in an enzymatic assay with beta-1,4-galactosyltransferase.