Effects of miRNA-197 overexpression on proliferation, apoptosis and migration in levonorgestrel treated uterine leiomyoma cells.
Effects of miRNA-197 overexpression on proliferation, apoptosis and migration in levonorgestrel treated uterine leiomyoma cells.
复制标题
miRNA-197 过表达对左炔诺孕酮治疗的子宫肌瘤细胞增殖、凋亡和迁移的影响。
DOI：
10.1016/j.biopha.2015.02.004
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发表时间：
2015-04
期刊：
影响因子：
--
通讯作者：
Xu, Qing
中科院分区：
文献类型：
--
作者：
Fu, Ziyi;Ji, Chenbo;Wu, Jiangping;Xu, Qing
子宫平滑肌瘤是女性生殖道最早的良性肿瘤，可导致月经异常、反复流产等严重的妇科疾病。近年来，随着对肿瘤发生机制的探索，microRNAs（miRNAs）受到了越来越多的关注。结果miR-197在体外可抑制子宫肌瘤细胞增殖，诱导细胞凋亡，阻断细胞迁移。与此同时，左炔诺孕酮（LNG）可以诱导人子宫平滑肌瘤细胞中miR-197的表达，过表达的miR-197与LNG对人子宫平滑肌瘤细胞的增殖和凋亡有协同作用。结论本研究数据显示miR-197在人子宫平滑肌瘤细胞中可以发挥抑癌作用，结论：miR-197可能是一个潜在的靶点，为临床治疗提供了数据库。
Background/aimsUterine leiomyoma is the ahead benign tumor of the female genital tract, which resulted in menstrual abnormalities, recurrent pregnancy loss, and other serious gynecological disorders in women. Recently, as the process of exploring the brief molecular mechanisms of tumorgenesis, microRNAs (miRNAs) have attracted much more attention.MethodsIn this study, we first confirmed that microRNA-197 (miR-197) was down-regulated significantly in human uterus leiomyoma by quantity real-time polymerase chain reaction, compared to normal uterus myometrium. Then we observed the potential effects of miR-197 overexpression on human uterus leiomyoma cells by cell counting kit 8, wound healing assay, and flow cytometric assessment separately.ResultsThe data showed that miR-197 could inhibit cell proliferation, induce cell apoptosis, and block cell migration in vitro. Coincidently, levonorgestrel (LNG), a well-known uterus leiomyoma therapy, could induce miR-197 expression in human uterus leiomyoma cells, and over-expression of miR-197 showed a synergy effect on human uterus leiomyoma cell proliferation and apoptosis with LNG.ConclusionIn this study, the data showed that miR-197 could play an anti-oncogenic role in human uterus leiomyoma cells, and cooperate with LNG on the cell proliferation and apoptosis, which suggested that miR-197 might be a potential target and provided database for clinical treatment.
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