Qualitative and quantitative analysis of chemical constituents in Ardisiae Japonicae Herba
Qualitative and quantitative analysis of chemical constituents in Ardisiae Japonicae Herba
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紫金牛化学成分的定性定量分析
DOI：
10.1002/jssc.201700667
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发表时间：
2017
影响因子：
3.1
通讯作者：
Liu E-Hu
中科院分区：
文献类型：
--
作者：
Yu Ke-Yun;Gao Wen;Li Shang-Zhen;Wu Wei;Li Pei;Dou Li-Li;Wang Yuan-Zhong;Liu E-Hu
紫金牛（Ardisiae Japonicae Herba）是一种著名的传统中药，用于治疗支气管炎、结膜炎、肺炎和创伤。本文首次建立了高效液相色谱-四极杆飞行时间质谱联用技术对紫金牛中的化学成分进行分离和结构鉴定。共15个化合物，包括香豆素类，黄酮苷类，儿茶素进行了鉴定或初步表征，根据其色谱行为和质谱碎片，并与参考标准进行比较。此外，一个简单的高效液相色谱-二极管阵列检测器的方法被开发用于同时测定五个主要成分。方法学验证的线性、精密度、重复性、稳定性和回收率结果表明，所建立的方法可靠、准确。岩白菜素和槲皮苷是紫金牛中含量最高的成分，可作为紫金牛质量控制的化学标记物。
Ardisiae Japonicae Herba is a well‐known traditional Chinese medicine for the treatment of bronchitis conjunctivitis, pneumonia, and trauma. In this work, a high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry method was first established for the separation and structural identification of the chemical constituents in Ardisiae Japonicae Herba. A total of 15 compounds including coumarins, flavonoid glycosides, and catechins were identified or tentatively characterized based on their chromatographic behaviors and mass spectral fragmentation and by comparisons with the reference standards. Furthermore, a simple high‐performance liquid chromatography with diode array detection method was developed for the simultaneous determination of five major constituents. Results obtained from method validation, including linearity, precision, repeatability, stability, and recovery, showed that the established method was reliable and accurate. Bergenin and quercitrin were found to be the most abundant constituents and could be served as chemical markers for quality control of Ardisiae Japonicae Herba.