Performance of the genotype MTBDR line probe assay for detection of resistance to rifampin and isoniazid in strains of Mycobacterium tuberculosis with low- and high-level resistance
Performance of the genotype MTBDR line probe assay for detection of resistance to rifampin and isoniazid in strains of Mycobacterium tuberculosis with low- and high-level resistance
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DOI：
10.1128/jcm.01054-06
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发表时间：
2006-10-01
影响因子：
9.4
通讯作者：
Sougakoff, Wladimir
中科院分区：
文献类型：
--
作者：
Brossier, Florence;Veziris, Nicolas;Sougakoff, Wladimir
我们评估了基因型MTBDR线性探针检测试剂盒的性能，该试剂盒通过检测rpoB和katG基因中最常见的突变，同时鉴定结核分枝杆菌及其对利福平（RIF）和异烟肼（INH）的耐药性。一百一十三米。测试结核分离株。还测定了katG和inhA基因以及mabA-inhA启动子区的核苷酸序列。MTBDR检测分别检出100%和67%（n = 64）的RIF和INH耐药菌株。在后者中，62株携带katG的Ser 315 Thr突变，其中59株显示出对INH的高水平耐药。2株INH低耐药菌株存在Ser 315 Asn突变。MTBDR检测未发现31株INH耐药菌株（33%）发生突变，其中24株显示低水平耐药。通过DNA测序，发现7株菌株KatG蛋白发生不同程度的突变，17株菌株mabA-inhA启动子-15位点发生C-T突变，7株菌株InhA基因发生Ser 94 Ala突变。总之，MTBDR测定法很容易适应常规实验室的工作流程，能够检测100%的RIF耐药菌株和89%的具有高水平耐药性的INH耐药菌株，但仅17%的菌株具有低水平的INH耐药性，表明该方法可作为一种快速检测利福平耐药和异烟肼高水平耐药菌株的方法。结核
We assessed the performance of the Genotype MTBDR line probe assay that offers the simultaneous identification of Mycobacterium tuberculosis and its resistance to rifampin (RIF) and isoniazid (INH) by detecting the most commonly found mutations in the rpoB and katG genes. One hundred thirteen M. tuberculosis isolates were tested. The nucleotide sequences of the katG and inhA genes and the mabA-inhA promoter region were also determined. The MTBDR assay detected 100% and 67% (n = 64) of the strains resistant to RIF and INH, respectively. Among the latter, 62 strains carried a Ser315Thr mutation in katG, 59 of them displaying a high level of resistance to INH. Two strains with a low level of INH resistance had a Ser315Asn mutation. No mutation was found by the MTBDR assay for 31 INH-resistant strains (33%), of which 24 showed a low level of resistance. By DNA sequencing, we found among them various mutations in the KatG protein for 7 strains, a C-T mutation in position - 15 of the mabA-inhA promoter in 17 strains, and a Ser94Ala mutation in InhA for 7 strains. In conclusion, the MTBDR assay, which fits easily in the workflow of a routine laboratory, enabled the detection of 100% of the RIF-resistant strains and 89% of the INH-resistant strains with a high level of resistance but only 17% of the strains characterized by a low level of INH resistance, indicating that the test can be used as a rapid method to detect in the same experiment the rifampin-resistant and the high-level isoniazid-resistant strains of M. tuberculosis.