Insulin-like growth factor-I enhances cell-based repair of articular cartilage
Insulin-like growth factor-I enhances cell-based repair of articular cartilage
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DOI：
10.1302/0301-620x.84b2.11167
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发表时间：
2002-03-01
影响因子：
--
通讯作者：
Nixon, AJ
中科院分区：
文献类型：
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作者：
Fortier, LA;Mohammed, HO;Nixon, AJ
在马软骨广泛丧失的模型中，在关节镜下修复全层软骨缺损期间，软骨细胞和聚合纤维蛋白的复合物添加了胰岛素样生长因子-I (IGF-I)。通过IGF-I和软骨细胞-纤维蛋白复合物促进的修复，或仅用软骨细胞-纤维蛋白复合物治疗的对照缺陷，在死亡前通过临床表现和滑液的重复分析进行比较，并在术后八个月终止时通过组织形态、胶原蛋白分型和生化测定进行比较。通过甲苯胺蓝反应以及I型和II型胶原原位杂交和免疫组织化学对软骨结构进行组织学评估。通过 DNA 测定、蛋白多糖定量和表征、反相高效液相色谱评估胶原蛋白、使用溴化氰消化进行胶原蛋白分型以及通过聚丙烯酰胺凝胶电泳进行肽分离来对修复组织进行生化评估。八个月时的结果表明，向软骨细胞移植物中添加 IGF-I 可以增强软骨细胞移植物的软骨形成。 软骨缺陷，包括融入周围软骨。缺损的总体填充得到改善，组织中产生 II 型胶原蛋白的细胞比例更高。 H 型胶原蛋白的测量显示 IGF-I 处理的缺损水平有所改善，支持对缺损进行原位杂交和免疫组织化学评估。 IGF-I 提高了大型全层修复模型中软骨细胞纤维蛋白移植物的修复能力。
Composites of chondrocytes and polymerised fibrin were supplemented with insulin-like growth factor-I (IGF-I) during the arthroscopic repair of full-thickness cartilage defects in a model of extensive loss of cartilage in horses. Repairs facilitated with IGF-I and chondrocyte-fibrin composites, or control defects treated with chondrocyte-fibrin composites alone, were compared before death by the clinical appearance and repeated analysis of synovial fluid, and at termination eight months after surgery by tissue morphology, collagen typing, and biochemical assays. The structure of cartilage was evaluated histologically by Toluidine Blue reaction and collagen type-I and type-II in situ hybridisation and immunohistochemistry. Repair tissue was biochemically evaluated by DNA assay, proteoglycan quantitation and characterisation, assessment of collagen by reverse-phase high-performance liquid chromatography, and collagen typing using cyanogen bromide digestion and peptide separation by polyacrylamide gel electrophoresis.The results at eight months showed that the addition of IGF-I to chondrocyte grafts enhanced chondrogenesis in cartilage defects, including incorporation into surrounding cartilage. Gross filling of defects was improved, and the tissue contained a higher proportion of cells producing type-II collagen. Measurements of collagen type H showed improved levels in IGF-I-treated defects, supporting in situ hybridisation and immunohistochemical assessments of the defects. IGF-I improves the repair capabilities of chondrocyte-fibrin grafts in large full-thickness repair models.