On-chip surface-based detection with nanohole arrays
On-chip surface-based detection with nanohole arrays
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DOI：
10.1021/ac070001a
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发表时间：
2007-06-01
影响因子：
7.4
通讯作者：
Brolo, Alexandre G.
中科院分区：
文献类型：
--
作者：
De Leebeeck, Angela;Kumar, L. K. Swaroop;Brolo, Alexandre G.
介绍了一种基于金膜纳米孔阵列的集成表面等离子体共振（SPR）化学和生物传感器的微流控装置。在实验室中广泛使用SPR进行表面分析尚未转化为微流体分析芯片平台，部分原因是与缩小光学器件和共反射模式操作所需的表面积相关的挑战。通过金属膜中的亚波长孔径的光传输的共振增强建议使用纳米孔阵列作为小型化的基于SPR的传感元件。这里介绍的设备利用纳米孔阵列的独特性能：基于表面的灵敏度;传输模式操作;相对较小的占地面积;和可重复性。在芯片上进行的概念验证测量表明，在阵列表面的折射率的微小变化的响应。每折射率单位的333 nm的灵敏度被证明与集成设备。该装置还被应用于检测空间微流体浓度梯度和监测涉及生物素-链霉亲和素系统的生化亲和过程。结果表明纳米孔阵列作为微流体平台中基于表面等离子体的传感元件的功效，为现有的基于微流体的分析工具套件增加了独特的表面敏感诊断能力。
A microfluidic device with integrated surface plasmon resonance (SPR) chemical and biological sensors based on arrays of nanoholes in gold films is demonstrated. Widespread use of SPR for surface analysis in laboratories has not translated to microfluidic analytical chip platforms, in part due to challenges associated with scaling down the optics and the surface area required for common reflection mode operation. The resonant enhancement of light transmission through subwavelength apertures in a metallic film suggests the use of nanohole arrays as miniaturized SPR-based sensing elements. The device presented here takes advantage of the unique properties of nanohole arrays: surface-based sensitivity; transmission mode operation; a relatively small footprint; and repeatability. Proof-of-concept measurements performed on-chip indicated a response to small changes in refractive index at the array surfaces. A sensitivity of 333 nm per refractive index unit was demonstrated with the integrated device. The device was also applied to detect spatial microfluidic concentration gradients and to monitor a biochemical affinity process involving the biotin-streptavidin system. Results indicate the efficacy of nanohole arrays as surface plasmon-based sensing elements in a microfluidic platform, adding unique surface-sensitive diagnostic capabilities to the existing suite of microfluidic-based analytical tools.