Isolation, chemical characterization, and quantitation of A beta 3-pyroglutamyl peptide from neuritic plaques and vascular amyloid deposits
Isolation, chemical characterization, and quantitation of A beta 3-pyroglutamyl peptide from neuritic plaques and vascular amyloid deposits
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DOI：
10.1006/bbrc.1997.7083
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发表时间：
1997-08-08
影响因子：
3.1
通讯作者：
Roher, AE
中科院分区：
文献类型：
--
作者：
Kuo, YM;Emmerling, MR;Roher, AE
从阿尔茨海默病患者大脑的神经性斑块和血管壁中，我们已经提纯并量化了一种Aβ肽。它以焦谷氨基(Aβ3pE)的形式从残基3Glu开始。神经炎斑块中N端截短的Aβ3pE占Aβ的51%。其次是从1Asp位置开始的30%，其中包括异构化形式(IsoAsp)的20%。相比之下，血管淀粉样蛋白平均只含有11%的Aβ3pE，主要成分从残基1Asp开始(69%)，其中只有6%以IsoAsp的形式存在。Aβ3pE的存在具有重要的结构后果，因为它比其他形式的Aβ更疏水，从而增加了Aβ的不溶性。此外，Aβ3pE与常见氨基肽酶作用的N端被阻断，可能导致Aβ在阿尔茨海默病的神经性斑块中大量积聚。(C)1997年学术出版社。
From the neuritic plaques and vascular walls of the brains of patients with Alzheimer disease, we have purified and quantified an A beta peptide. which starts at residue 3Glu in the form of pyroglutamyl (A beta 3pE). The N-terminally truncated A beta 3pE comprised 51% of the A beta in the neuritic plaques. This was followed by 30% starting at position 1Asp which included 20% in the isomerized form (IsoAsp). In contrast, the vascular amyloid only contained an average of 11% in the form of A beta 3pE with the major component starting at residue 1Asp (69%), which included only 6% in the form of IsoAsp. The presence of A beta 3pE has important structural consequences since it is more hydrophobic than other forms of A beta, thus increasing the insolubility of A beta. In addition, A beta 3pE, with its blocked N-terminus to the action of common aminopeptidases, may result in the profuse accumulation of A beta in the neuritic plaques of Alzheimer disease. (C) 1997 Academic Press.