Profiling of miRNAs in porcine Sertoli cells
Profiling of miRNAs in porcine Sertoli cells
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DOI：
10.1186/s40104-020-00487-6
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发表时间：
2020-08-17
影响因子：
7
通讯作者：
Zeng, Wenxian
中科院分区：
文献类型：
--
作者：
Chen, Xiaoxu;Zheng, Yi;Zeng, Wenxian
背景：支持细胞（Sertoli cells，SC）是支持和支配精子发生的特殊环境. MicroRNAs（miRNAs）是一类长度为22 nt的小分子非编码RNA，在小鼠精子干细胞中含量丰富，在精子发生过程中起重要作用。然而，猪干细胞的miRNAs在很大程度上仍然未知。通过比较生殖细胞中的miRNA，系统分析了猪SC中miRNA的表达模式。我们筛选了高度富集的SC miRNAs，并通过Gene Ontology分析预测了它们的功能。结果：18个miRNAs在SC中高表达，15个miRNAs在生殖细胞中高表达。预测这些miRNAs介导SC和生殖细胞功能。结论：本研究为猪SC的miRNA表达模式及其调控机制提供了新的视角。
Background: Sertoli cells (SCs) create a specialized environment to support and dictate spermatogenesis. MicroRNAs (miRNAs), a kind of similar to 22 nt small noncoding RNAs, have been reported to be highly abundant in mouse SCs and play critical roles in spermatogenesis. However, the miRNAs of porcine SCs remain largely unknown.Methods: We isolated porcine SCs and conducted small RNA sequencing. By comparing miRNAs in germ cells, we systematically analyzed the miRNA expression pattern of porcine SCs. We screened the highly enriched SC miRNAs and predicted their functions by Gene Ontology analysis. The dual luciferase assay was used to elucidate the regulation of tumor necrosis factor receptor (TNFR)-associated factor 3 (TRAF3) byssc-miR-149.Results: The analysis showed that 18 miRNAs were highly expressed in SCs and 15 miRNAs were highly expressed in germ cells. These miRNAs were predicted to mediate SC and germ cell functions. In addition,ssc-miR-149played critical roles in SCs by targetingTRAF3.Conclusion: Our findings provide novel insights into the miRNA expression pattern and their regulatory roles of porcine SCs.