Controlling Size and Surface Chemistry of Cationic Nanogels by Inverse Microemulsion ATRP
Controlling Size and Surface Chemistry of Cationic Nanogels by Inverse Microemulsion ATRP
复制标题
通过反相微乳液 ATRP 控制阳离子纳米凝胶的尺寸和表面化学
DOI：
10.1002/macp.202200210
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发表时间：
2022
影响因子：
2.5
通讯作者：
Matyjaszewski, Krzysztof
中科院分区：
文献类型：
--
作者：
Simakova, Antonina;Averick, Saadyah;Jazani, Arman Moini;Matyjaszewski, Krzysztof
阳离子纳米凝胶的独特性质，如其亲水性和高负载能力，使其成为一种有前途的药物递送剂平台，特别是用于递送亲水性生物分子。尽管存在几种阳离子纳米凝胶的合成方法，但在分散介质中聚合是有利的，因为其能够提供对组成的控制和高单体转化率。然而，由于奥斯特瓦尔德熟化过程，聚合物液滴通常在聚合期间遭受尺寸的显著增加。本文报道了在亲水性引发剂单体的反相微乳液条件下通过原子转移自由基聚合制备阳离子纳米凝胶，所述亲水性引发剂单体防止单体扩散并因此限制聚合期间液滴的生长。此外，纳米凝胶的表面官能度可以通过应用疏水反应性表面活性剂或通过接枝亲水性壳以形成核-壳阳离子纳米凝胶来调节。合成的阳离子纳米凝胶是生物相容的，内化到HEK 293细胞中，并且对质粒DNA具有高络合能力。
The unique properties of cationic nanogels, such as their hydrophilicity and high loading capacity, make them a promising platform as drug delivery agents, particularly for the delivery of hydrophilic biomolecules. Although several synthetic methods exist for cationic nanogels, polymerization in dispersed media is advantageous due to its ability to provide control over composition and high monomer conversion. However, polymer droplets typically suffer from a significant increase in size during polymerization due to the Ostwald ripening process. Herein, the preparation of cationic nanogels by atom transfer radical polymerization under inverse microemulsion conditions of a hydrophilic inimer that prevents monomer diffusion and hence limits droplets’ growth during polymerization is reported. Additionally, the surface functionality of the nanogels can be modulated by the application of hydrophobic reactive surfactants or by grafting hydrophilic shells to form core‐shell cationic nanogels. The synthesized cationic nanogels are biocompatible, internalized to HEK 293 cells, and have a high complexation ability for plasmid DNA.