Changes in the actinomycetal communities during continuous thermophilic composting as revealed by denaturing gradient gel electrophoresis and quantitative PCR.
Changes in the actinomycetal communities during continuous thermophilic composting as revealed by denaturing gradient gel electrophoresis and quantitative PCR.
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DOI：
10.1016/j.biortech.2010.09.034
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发表时间：
2011
影响因子：
11.4
通讯作者：
Yong Xiao;G. Zeng;Zhao-hui Yang;Yanqing Ma;Cui Huang;Zheng-yong Xu;Jing Huang;Chang-Zheng Fan-Chang-Zheng-F
中科院分区：
文献类型：
--
作者：
Yong Xiao;G. Zeng;Zhao-hui Yang;Yanqing Ma;Cui Huang;Zheng-yong Xu;Jing Huang;Chang-Zheng Fan-Chang-Zheng-F
放线菌在堆肥过程中降解纤维素和溶解木质素。采用变性梯度凝胶电泳（DGGE）和定量PCR（qPCR）技术，研究了连续高温堆肥（CTC）和传统堆肥（TC）过程中放线菌群落多样性和放线菌16 SrDNA拷贝数的变化。qPCR结果表明，在活性堆肥的相似阶段，CTC样品的基因拷贝数比TC样品高25-80%，而在两个运行的成熟堆肥中，CTC样品的基因拷贝数均低于3× 109拷贝/g（干重）。DGGE结果表明，在峰后温度阶段，CTC的放线菌群落比TC的多样性更高，平均为16条带，而TC为12条带。研究表明，CTC中高于50°C的温度有利于放线菌的生长。
Actinomycetes degrade cellulose and solubilize lignin during composting. Changes in the diversity of the actinomycetal communities and the 16S rDNA copy numbers of actinomycetes were monitored by denaturing gradient gel electrophoresis (DGGE) and quantitative PCR (qPCR), respectively, during continuous thermophilic composting (CTC) and traditional composting (TC). qPCR indicated that the copy numbers from the CTC samples were 25–80% higher than those from the TC samples during similar phases of active composting and they were lower than 3×109gene copies/g (dry weight) in the mature compost from both runs. DGGE showed a more diverse actinomycetal community in the CTC than in TC, averaging 16 bands as compared to 12 bands, at the post peak temperature phase. The study suggested that temperatures higher than 50°C in CTC benefited the growth of actinomycetes.