Expression patterns of Fgf8 and Shh in the developing external genitalia of Suncus murinus
Expression patterns of Fgf8 and Shh in the developing external genitalia of Suncus murinus
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DOI：
10.1530/rep-16-0231
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发表时间：
2017-02-01
期刊：
影响因子：
3.8
通讯作者：
Oda, Sen-ichi
中科院分区：
文献类型：
--
作者：
Miyado, Mami;Miyado, Kenji;Oda, Sen-ichi
生殖结节（GT）是外生殖器的胚胎原基，其发育受上皮-间质相互作用和多种信号通路的调控。亚洲家麝鼩 Suncus murinus（以下，实验室名称：Suncus）属于Eulipotyphla（前食虫目或Soricomorpha）的成年雄性外生殖器的形态不同于小鼠和人类。然而，对向日葵GT的发育过程及其调控基因尚不清楚。在本研究中，我们探讨了太阳花GT发育过程中的形态变化和基因表达模式。（在最初的生长过程中）和物种特异性在基因表达分析中，成纤维细胞生长因子8（Fgf 8）和音刺猬（Shh），分别是小鼠GT发育的指示剂和调节剂，结果表明，FGF和SHH信号在GT的形成过程中起着重要的调控作用，其表达模式与发育中的小鼠GT相似在哺乳动物中广泛保守。
Reciprocal epithelial-mesenchymal interactions and several signalling pathways regulate the development of the genital tubercle (GT), an embryonic primordium of external genitalia. The morphology of the adult male external genitalia of the Asian house musk shrew Suncus murinus (hereafter, laboratory name: suncus) belonging to the order Eulipotyphla (the former order Insectivora or Soricomorpha) differs from those of mice and humans. However, the developmental process of the suncus GT and its regulatory genes are unknown. In the present study, we explored the morphological changes and gene expression patterns during the development of the suncus GT. Morphological observations suggested the presence of common (during the initial outgrowth) and species-specific (during the sexual differentiation of GT) developmental processes of the suncus GT. In gene expression analysis, fibroblast growth factor 8 (Fgf8) and sonic hedgehog (Shh), an indicator and regulator of GT development in mice respectively, were found to be expressed in the cloacal epithelium and the developing urethral epithelium of the suncus GT. This pattern of expression specifically in GT epithelium is similar to that observed in the developing mouse GT. Our results indicate that the mechanism of GT formation regulated by the FGF and SHH signalling pathways is widely conserved in mammals.