The prevalence of human parvovirus B19 DNA and antibodies in blood donors from four Chinese blood centers
The prevalence of human parvovirus B19 DNA and antibodies in blood donors from four Chinese blood centers
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DOI：
10.1111/j.1537-2995.2011.03067.x
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发表时间：
2011-09-01
期刊：
影响因子：
2.9
通讯作者：
Li, Wuping
中科院分区：
文献类型：
--
作者：
Ke, Ling;He, Miao;Li, Wuping
背景：人类细小病毒B19是一种常见的人类病原体，可导致从无症状到严重的各种疾病，尤其是在免疫功能低下的患者中。B19病毒可通过血液和/或血液制品传播，其对常见病毒灭活和/或清除方法的抵抗力提高了与B19相关的血液安全的重要性。然而，B19在中国献血员中的存在、变异和载量尚未确定。研究设计和方法：建立定量聚合酶链式反应(PCR)检测血浆标本中三种人类红细胞病毒DNA的方法。通过实时微池核酸扩增技术(NAT)对来自中国四个血液中心的3957名献血者进行了B19基因筛查。然后对阳性样本进行套式聚合酶链式反应确认，并进行序列分析和比对以进行系统发育研究。结果：3957例献血员中，23例(0.58%)B19 DNA阳性。DNA定量水平为2.48×10(2)~6.38×10(4)拷贝/毫升。系统发育树分析表明，中国献血员中流行的基因类型为B19基因1型。对448例中国献血员进行了B19抗体检测，其中B19抗体阳性率为24.6%，IgM抗体阳性率为6.9%。有2.5%的样本两种抗体亚型均为阳性。结论：中国是否应开展血液和血液制品的B19 NAT筛查，还需要进行更大规模的研究，以便做出明智的决定。
BACKGROUND: Human parvovirus B19 is a common human pathogen that causes a variety of diseases with outcomes ranging from asymptomatic to severe, especially in immunocompromised patients. The B19 virus can be transmitted via blood and/or blood products and its resistance to common viral inactivation and/or removal methods raises the importance of B19-related blood safety. However, the existence, variation, and loading of B19 in Chinese blood donors have not been determined.STUDY DESIGN AND METHODS: Quantitative polymerase chain reaction (PCR) was developed to detect all three genotypes of the human erythrovirus DNA in plasma samples. In total, 3957 donations from four Chinese blood centers were screened for B19 by real-time minipool nucleic acid amplification technology (NAT). The positive samples were then confirmed by nested PCR and subjected to sequence analysis and alignment for phylogenetic studies. An enzyme-linked immunosorbent assay-based experiment was also performed to identify the prevalence of immunoglobulin (Ig)G and/or IgM antibodies specific to the B19 structural proteins in acquired samples.RESULTS: Of 3957 blood donors, 23 (0.58%) specimens were found positive for B19 DNA. The quantitative DNA levels ranged from 2.48 x 10(2) to 6.38 x 10(4) copies/mL. The phylogenic analyses showed that the prevalent genotypes in Chinese blood donors belong to B19 Genotype 1. A total of 448 samples from Chinese blood donors were investigated for the seroprevalence of B19 antibodies, among which 24.6 and 6.9% of specimens were seropositive for B19 IgG and IgM antibodies, respectively. A total of 2.5% of these samples were positive for both antibody isotypes.CONCLUSIONS: Whether B19 NAT screening of blood and blood products should be launched in China, larger studies are needed to facilitate an informed decision.