Regulation of BSF-2/IL-6 production by human mononuclear cells. Macrophage-dependent synthesis of BSF-2/IL-6 by T cells.
Regulation of BSF-2/IL-6 production by human mononuclear cells. Macrophage-dependent synthesis of BSF-2/IL-6 by T cells.
复制标题
人单核细胞产生 BSF-2/IL-6 的调节。
作者：
Y. Horii;A. Muraguchi;S. Suematsu;Tadashi Matsuda;K. Yoshizaki;T. Hirano;T. Kishimoto
研究了外周血单个核细胞(MNC)分泌BSF-2/IL-6的调节。在丝裂原刺激的MNC中，BSF-2mRNA的表达呈现双相反应，第一个高峰在4h左右，第二个高峰在48h左右，这是由于不同亚群的MNC表达BSF-2mRNA的动力学不同所致，M Phi在没有任何刺激的情况下在5h表达BSF-2mRNA，在丝裂原刺激的T细胞和B细胞在刺激后48h表达BSF-2mRNA。抗BSF-2抗体免疫组织化学染色显示巨噬细胞、T细胞和B细胞均可产生BSF-2。外周MNC中的T细胞在M phi存在的情况下产生BSF-2。巨噬细胞对T细胞产生BSF-2的需求可被TPA取代，但不能被IL-1或BSF-2所取代。
Regulation of BSF-2/IL-6 production in peripheral mononuclear cells (MNC) was studied. BSF-2 mRNA expression in mitogen-stimulated MNC showed a biphasic response, the first peak around 4 h and the second peak around 48 h. This was caused by different kinetics of BSF-2 mRNA expression in distinct subpopulations of MNC; M phi expressed BSF-2 mRNA at 5 h in the absence of any stimulation, and mitogen-stimulated T cells and B cells expressed BSF-2 mRNA 48 h after stimulation. Immunohistochemical staining of the cells with anti-BSF-2 antibody demonstrated that macrophages, T cells and B cells could produce BSF-2. T cells in peripheral MNC produced BSF-2 in the presence of M phi. The requirement of macrophage for BSF-2 production in T cells could be replaced by TPA but not by IL-1 or BSF-2.