Cumulus cell contact during oocyte maturation in mice regulates meiotic spindle positioning and enhances developmental competence
Cumulus cell contact during oocyte maturation in mice regulates meiotic spindle positioning and enhances developmental competence
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DOI：
10.1007/s10815-009-9376-9
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发表时间：
2010-01-01
影响因子：
3.1
通讯作者：
Albertini, David F.
中科院分区：
文献类型：
--
作者：
Barrett, Susan L.;Albertini, David F.
为探讨卵丘细胞在卵母细胞成熟过程中的接触对减数分裂纺锤体组装和发育能力获得的作用，采用共聚焦显微镜对小鼠卵巢体外成熟和体内成熟的卵丘卵母细胞复合体进行了分析，观察了卵母细胞在胚胎培养过程中的体细胞接触情况和孤雌激活后的发育能力。诱发卵母细胞皮质减数分裂纺锤体组装和发育能力获得。相反，在体外成熟的卵母细胞失去细胞接触与中央减数分裂纺锤体组装一致，导致卵裂延迟卵激活和未能形成囊胚。肌动蛋白解聚剂latrunculin B对细胞接触的实验性破坏导致形成具有分散的染色体的扩大的减数分裂纺锤体，这与在体内成熟后形成的纺锤体上观察到的染色体的紧凑排序不同，提示细胞接触与发育能力的获得之间存在联系。在减数分裂成熟过程中，体细胞接触通过调节卵母细胞的空间组织和功能来优化卵母细胞的质量。减数分裂纺锤体通过肌动蛋白依赖的机制，促进发展。
To investigate the role of cumulus cell contact during oocyte maturation on meiotic spindle assembly and the acquisition of developmental competence.Cumulus oocyte complexes isolated from mouse ovaries subjected to in vitro or in vivo maturation were analyzed by confocal microscopy with respect to oocyte somatic cell contacts and for their ability to develop after parthenogenic activation during embryo culture.Cell contact is maintained during maturation in vivo, predisposing oocytes to cortical meiotic spindle assembly and developmental competence acquisition. In contrast, oocytes matured in vitro lose cell contact coincident with central meiotic spindle assembly that results in cleavage delays upon egg activation and failure to form blastocysts. Experimental disruption of cell contact by the actin-depolymerizing agent latrunculin B results in the formation of enlarged meiotic spindles with dispersed chromosomes unlike the compact ordering of chromosomes observed on spindles formed after in vivo maturation, suggesting a link between cell contact and the acquisition of developmental competence.Somatic cell contact optimizes oocyte quality during meiotic maturation by regulating the spatial organization and function of the meiotic spindle through actin-dependent mechanisms that enhance development.