Overexpression of HOXA10 promotes gastric cancer cells proliferation and HOXA10+/CD44+ is potential prognostic biomarker for gastric cancer
Overexpression of HOXA10 promotes gastric cancer cells proliferation and HOXA10+/CD44+ is potential prognostic biomarker for gastric cancer
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HOXA10过表达促进胃癌细胞增殖，HOXA10/CD44是胃癌潜在的预后生物标志物
DOI：
10.1016/j.ejcb.2015.08.004
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发表时间：
2015-12-01
影响因子：
6.6
通讯作者：
Zhou, Chong-zhi
中科院分区：
文献类型：
--
作者：
Han, Yang;Lu, Su;Zhou, Chong-zhi
胃癌（GC）是一种预后较差的恶性肿瘤。本研究旨在探讨同源框A10（HOXA10）在GC中的作用以及HOXA10/CD44表达与GC预后的相关性。基于 50 对新鲜 GC 样本和邻近正常样本的 qRT-PCR 和 Western Blot 分析，发现 HOXA10 在 GC 组织中的 mRNA 和蛋白质水平显着上调。 HOXA10过表达的GC细胞中细胞增殖、迁移和侵袭得到增强，而HOXA10沉默的细胞中细胞增殖、迁移和侵袭受到抑制。通过IPA软件预测HOXA10通过MSN与CD44相互作用，并通过Western Blot初步证实。通过免疫组化和组织微阵列（N = 264）发现HOXA10表达与肿瘤大小（P=0.011）和CD44表达显着相关（P
Gastric cancer (GC) is a malignant cancer with poor prognosis. This study aims to investigate the roles of homeobox A10 (HOXA10) in GC and the correlations between HOXA10/CD44 expression and GC prognosis. Based on qRT-PCR and Western Blot analyses in 50 pairs of fresh GC samples and adjacent normal samples, it is identified that HOXA10 was significantly up-regulated in GC tissues at mRNA and protein levels. Cell proliferation, migration, and invasion were enhanced in GC cells with overexpressed HOXA10, while inhibited in cells with silenced HOXA10. Through IPA software, HOXA10 was predicted to interact with CD44 via MSN, which was preliminarily confirmed by using Western Blot. Through immunohistochemistry and tissue microarray (N = 264), it is found that HOXA10 expression was significantly correlated with tumor size (P=0.011) and CD44 expression (P