Evidence for lipopolysaccharide-induced differentiation of RAW264-7 murine macrophage cell line into dendritic like cells
Evidence for lipopolysaccharide-induced differentiation of RAW264-7 murine macrophage cell line into dendritic like cells
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DOI：
10.1007/bf02970143
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发表时间：
2003-02-01
影响因子：
2.9
通讯作者：
Lewis, DM
中科院分区：
文献类型：
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作者：
Saxena, RK;Vallyathan, V;Lewis, DM
研究了脂多糖（LPS）对RAW264.7巨噬细胞的影响。lps处理的RAW264.7细胞体积增大，树突形态明显。在LPS的最佳剂量（1杯/毫升）下，几乎70%的RAW264.7细胞获得树突形态。流式细胞术研究表明，已知在树突状细胞上表达并参与抗原呈递和T细胞活化的细胞表面标志物（B7.1、B7.2、CD40、MHC II类抗原和CD1d）在lps处理的RAW264.7细胞上也显著上调。我们的结果提示LPS本身可能构成巨噬细胞向dc样细胞分化的充分信号。
Effect of lipopolysaccharide (LPS) on RAW264.7 macrophage cell line was studied. LPS-treated RAW264.7 cells increased in cell size and acquired distinct dendritic morphology. At the optimal dose of LPS (1 mug/ml), almost 70% RAW264.7 cells acquired dendritic morphology. Flow cytometric studies indicate that the cell surface markers known to be expressed on dendritic cells and involved in antigen presentation and T cell activation (B7.1, B7.2, CD40, MHC class II antigens and CD1d) were also markedly upregulated on LPS-treated RAW264.7 cells. Our results suggest the possibility that LPS by itself could constitute a sufficient signal for differentiation of macrophages into DC-like cells.