Allometry of cell types in planarians by single cell transcriptomics
Allometry of cell types in planarians by single cell transcriptomics
复制标题
DOI：
10.1101/2023.11.01.565140
复制
发表时间：
2023-11
期刊：
影响因子：
--
通讯作者：
Elena Emili;Alberto Pérez-Posada;Marika Christodoulou;Jordi Solana
中科院分区：
文献类型：
--
作者：
Elena Emili;Alberto Pérez-Posada;Marika Christodoulou;Jordi Solana
异速生长法探讨了生物体的体型与其各种组成部分之间的关系，为生态学、生理学、新陈代谢和疾病提供了见解。细胞是生物系统的基本单位，但对细胞类型异速生长的研究相对较少。单细胞RNA测序（scRNA-seq）为研究细胞类型异速发育提供了一种很有前途的工具。能够按照异速生长缩放规则生长和去生长的涡虫，为这类研究提供了一个极好的模型。我们使用scRNA-seq检测了不同大小的无性涡虫的细胞类型异位，发现它们由相同的基本细胞类型组成，但比例不同。值得注意的是，肠道基底细胞对大小的变化最敏感，这表明它们在能量储存中起着重要作用。我们捕捉到不同细胞类型的基因调控程序对大小的反应。这项研究揭示了涡虫细胞类型异速变化的分子和细胞方面，并强调了scRNA-seq在此类研究中的实用性。
Allometry explores the relationship between an organism’s body size and its various components, offering insights into ecology, physiology, metabolism, and disease. The cell is the basic unit of biological systems, and yet, the study of cell type allometry remains relatively unexplored. Single-cell RNA sequencing (scRNA-seq) provides a promising tool for investigating cell type allometry. Planarians, capable of growing and degrowing following allometric scaling rules, serve as an excellent model for such studies. We used scRNA-seq to examine cell type allometry in asexual planarians of different sizes, revealing that they consist of the same basic cell types but in varying proportions. Notably, the gut basal cells are the most responsive to changes in size, suggesting a role in energy storage. We capture the gene regulatory programs of distinct cell types in response to size. This research sheds light on the molecular and cellular aspects of cell type allometry in planarians and underscores the utility of scRNA-seq in such investigations.