Changes in TRPC channel expression during postnatal development of cerebellar neurons
Changes in TRPC channel expression during postnatal development of cerebellar neurons
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DOI：
10.1016/j.ceca.2006.11.002
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发表时间：
2007-07-01
期刊：
影响因子：
4
通讯作者：
Glitsch, M. D.
中科院分区：
文献类型：
--
作者：
Huang, W.-C.;Young, J. S.;Glitsch, M. D.
在大脑中，经典（规范）瞬时受体电位（TRPC）通道被认为参与神经元发育的不同方面。我们研究了TRPC通道在大鼠出生后6周内小脑的发育表达谱。在这段时间内，TRPC3的表达显著上调，而TRPC4和TRPC6的表达显著下调。TRPC3主要在浦肯野细胞及其树突上表达，提示TRPC3表达的增加反映了浦肯野细胞树突树的发育。TRPC4的表达仅限于颗粒及其前体细胞。TRPC6在浦肯野细胞体、内颗粒细胞层的成熟颗粒细胞（不包括其前体）和分子层的中间神经元上均有表达。TRPC4表达的减少表明它是颗粒细胞发育所必需的，而TRPC6表达的减少可能与中间神经元的发育有关。此外，我们证明浦肯野细胞树突上存在功能性TRPC通道，这些通道在代谢性谷氨酸受体的刺激下被激活。我们的研究结果揭示了不同TRPC蛋白的细胞特异性表达模式，并提示TRPC蛋白表达的发育变化可能是出生后小脑正常发育所必需的。(C) 2006 Elsevier Ltd版权所有。
In the brain, classical (canonical) transient receptor potential (TRPC) channels are thought to be involved in different aspects of neuronal development. We investigated the developmental expression profile of TRPC channels in rat cerebellum during the first 6 weeks after birth. TRPC3 expression is significantly up-regulated whereas TRPC4 and TRPC6 expression are significantly down-regulated over this period of time. TRPC3 expression is mainly found on Purkinje cells and their dendrites, suggesting that the increase in TRPC3 expression reflects development of the dendritic tree of Purkinje cells. TRPC4 expression was restricted to granule and their precursor cells. TRPC6 expression is found on Purkinje cell bodies, on mature granule cells in the internal granule cell layer (but not their precursors) and interneurons in the molecular layer. The decrease in TRPC4 expression suggests that it is required for proper granule cell development whereas the decrease in TRPC6 expression is presumably correlated with interneuron development. Moreover, we demonstrate the presence of functional TRPC channels on Purkinje cell dendrites that are activated following stimulation of metabotropic glutamate receptors. Our results reveal cell-specific expression patterns for different TRPC proteins and suggest that developmental changes in TRPC protein expression may be required for proper postnatal cerebellar development. (C) 2006 Elsevier Ltd. All rights reserved.