Tricellulin expression in normal and neoplastic human pancreas
Tricellulin expression in normal and neoplastic human pancreas
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DOI：
10.1111/j.1365-2559.2012.04189.x
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发表时间：
2012-05-01
期刊：
影响因子：
6.4
通讯作者：
Kiss, Andras
中科院分区：
文献类型：
--
作者：
Korompay, Anna;Borka, Katalin;Kiss, Andras
目的：Tricellulin是紧密连接蛋白家族的一员，主要集中在三细胞接触处。在癌变过程中观察到一些紧密连接成分的表达改变。在本研究中，我们分析了三胞蛋白在正常人胰腺、原发性外分泌和内分泌胰腺肿瘤中的表达。方法与结果：共96例：正常胰腺20例，胰腺导管腺癌58例，胰腺内分泌肿瘤15例，腺泡细胞癌3例。进行免疫组织化学（数字形态学分析）、免疫荧光、免疫印迹分析和逆转录聚合酶链反应。三胞蛋白位于正常导管和腺泡的顶端，在三细胞接触处呈密集的、点状的免疫阳性，而在两个细胞之间的连接处观察到较弱的信号。朗格汉斯岛呈阴性。与低分化腺癌相比，高分化导管腺癌显著过表达三胞蛋白。腺泡细胞癌在肿瘤细胞中表达三胞蛋白。所有内分泌肿瘤均为三胞蛋白阴性。结论：这是第一个描述正常和肿瘤人胰腺中三胞蛋白表达谱的报告。正常和肿瘤胰腺外分泌组织均表达三胞蛋白，而在正常或肿瘤内分泌细胞中未见表达。胰腺导管腺癌中Tricellulin的表达与分化程度呈显著负相关。
Aims: Tricellulin is a member of the family of tight junction proteins, which are found concentrated mainly at tricellular contacts. Altered expression of several tight junction components has been observed during carcinogenesis. In the present study, we have analysed the expression of tricellulin in normal human pancreas, and in primary exocrine and endocrine pancreatic tumours.Methods and results: A total of 96 cases were studied: 20 normal pancreas, 58 pancreatic ductal adenocarcinomas, 15 pancreatic endocrine neoplasms, and three acinar cell carcinomas. Immunohistochemistry (analysed by digital morphometry), immunofluorescence, western blot analysis and reverse transcription polymerase chain reaction were performed. Tricellulin was localized apically in normal ducts and acini as intensive, spotty immunopositivity at tricellular contacts, whereas weaker signals were observed at the junction between two cells. Islets of Langerhans were negative. Well-differentiated ductal adenocarcinomas significantly overexpressed tricellulin as compared with poorly differentiated adenocarcinomas. Acinar cell carcinomas expressed tricellulin in tumour cells. All endocrine tumours were tricellulin-negative.Conclusions: This is the first report to describe the tricellulin expression profile in normal and neoplastic human pancreas. Both normal and neoplastic pancreatic exocrine tissues expressed tricellulin, whereas no expression was seen in normal or neoplastic endocrine cells. Tricellulin expression in pancreatic ductal adenocarcinomas showed a significant negative correlation with the degree of differentiation.