A FUNCTIONAL-ANALYSIS OF THE AGE-RELATED DEGENERATION IN THE FISCHER-344 RAT
A FUNCTIONAL-ANALYSIS OF THE AGE-RELATED DEGENERATION IN THE FISCHER-344 RAT
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DOI：
10.3109/02713689509033530
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发表时间：
1995-04-01
影响因子：
2
通讯作者：
DELCERRO, M
中科院分区：
文献类型：
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作者：
DILORETO, D;ISON, JR;DELCERRO, M
我们先前已经描述了如何表达感光细胞变性的Fischer 344大鼠受年龄，视网膜地形图，和性别。在动物的整个生命周期中，中央和赤道区域的退化随着年龄的增长而线性发展，而雄性动物的外周在12个月后的上级半球和18个月后的下半球中遭受突然和急剧的细胞损失。本研究的目的是确定这种变性如何影响3至24个月龄的雄性Fischer 344的视网膜功能和视觉能力。功能测试包括视网膜电图（ERG，测量a波和b波振幅和内隐时间）和惊吓反射修正的行为方法（RM，测量闪光抑制对紧接着的大声噪音的反应的程度）。所有的功能性措施都表现出随着年龄的增长而下降，但在其时间过程中有所不同。ERG振幅在整个年龄范围内呈线性下降。相比之下，ERG波的隐式时间和闪光抑制惊吓反应的程度都显示出从8个月或12个月大开始的功能轻微成熟（更快的隐式时间和更大的抑制）。18月龄后，内隐时间显著增加，惊吓反应显著减少。这项研究显示了视功能如何与年龄相关的视网膜变性的组织病理学变化相关。ERG内隐时间和反射抑制的变化的时间过程可能表明视网膜周边对这些措施的贡献。该研究表明多个功能终点在动物模型中评价视网膜疾病中的有用性。
We have previously described how the expression of photoreceptor cell degeneration in the Fischer 344 rat is affected by age, retinal topography, and gender. Degeneration in the central and equatorial regions progresses linearly with age throughout the life span of the animal, while the periphery of the male is subject to sudden and dramatic losses of cells in the superior hemisphere after 12 months and in the inferior hemisphere after 18 months of age. The purpose of the present study was to determine how this degeneration affected retinal function and visual ability in the male Fischer 344 from 3 to 24 months of age. Functional testing included the electroretinogram (ERG, measuring both a-wave and b-wave amplitudes and implicit times) and the behavioral method of startle reflex modification (RM, which measures the degree to which a light flash inhibits the response to an immediately subsequent loud noise). All of the functional measures showed a decline with age, but varied in their time course. ERG amplitudes showed a linear decline in amplitude over the entire age range. In contrast, the implicit times of the ERG waves and the degree to which the light flash inhibited the startle reaction both showed a slight maturation in function (faster implicit times and greater inhibition) from 8 or 12 months of age. After 18 months of age, the implicit time showed a significant increase and the startle response showed a significant decrease. This study shows how visual function correlates with the histopathological changes seen in age related retinal degeneration. The temporal course of the changes in ERG implicit times and reflex inhibition may indicate the contribution of the retinal periphery to these measures. The study suggests the usefulness of multiple functional endpoints in the evaluation of retinal disease in animal models.