Comprehensive Analysis of Rice Laccase Gene (OsLAC) Family and Ectopic Expression of OsLAC10 Enhances Tolerance to Copper Stress in Arabidopsis.
Comprehensive Analysis of Rice Laccase Gene (OsLAC) Family and Ectopic Expression of OsLAC10 Enhances Tolerance to Copper Stress in Arabidopsis.
复制标题
水稻漆酶基因 (OsLAC) 家族综合分析及 OsLAC10 异位表达增强拟南芥对铜胁迫的耐受性
DOI：
10.3390/ijms18020209
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发表时间：
2017-01-30
影响因子：
5.6
通讯作者：
Zheng L
中科院分区：
文献类型：
--
作者：
Liu Q;Luo L;Wang X;Shen Z;Zheng L
漆酶是由一个多基因家族编码的，广泛分布于植物基因组中，它们在植物发育和逆境反应中扮演着氧化单木醇产生高阶木质素的角色。我们从水稻中鉴定了30个漆酶基因，可分为5个亚家族，主要在胚乳、生长的根和茎的早期发育中表达。OsLACs可被激素、盐、干旱和重金属胁迫诱导。经20μM-Cu处理12h后，OsLAC10的表达水平提高了1200倍，并在大肠杆菌表达系统中证实了其漆酶活性。与野生型对照相比，过表达OsLAC10(OsLAC10-ox)的拟南芥根部木质素积累增加。OsLAC10-OX系在含铜毒害水平的1/2 Murashige和Skoog(MS)培养基上生长7天后，根显著长于野生型。与对照相比，水培条件下OsLAC10-OX根部铜浓度显著降低。这些结果为研究OsLAC家族的进化扩展和功能分化提供了新的视角。此外，OsLAC10可能参与木质素的生物合成，并减少拟南芥对铜的吸收，从而形成对铜的耐受性。
Laccases are encoded by a multigene family and widely distributed in plant genomes where they play roles oxidizing monolignols to produce higher-order lignin involved in plant development and stress responses. We identified 30 laccase genes (OsLACs) from rice, which can be divided into five subfamilies, mostly expressed during early development of the endosperm, growing roots, and stems. OsLACs can be induced by hormones, salt, drought, and heavy metals stresses. The expression level of OsLAC10 increased 1200-fold after treatment with 20 μM Cu for 12 h. The laccase activities of OsLAC10 were confirmed in an Escherichia coli expression system. Lignin accumulation increased in the roots of Arabidopsis over-expressing OsLAC10 (OsLAC10-OX) compared to wild-type controls. After growth on 1/2 Murashige and Skoog (MS) medium containing toxic levels of Cu for seven days, roots of the OsLAC10-OX lines were significantly longer than those of the wild type. Compared to control plants, the Cu concentration decreased significantly in roots of the OsLAC10-OX line under hydroponic conditions. These results provided insights into the evolutionary expansion and functional divergence of OsLAC family. In addition, OsLAC10 is likely involved in lignin biosynthesis, and reduces the uptake of Cu into roots required for Arabidopsis to develop tolerance to Cu.