Evidence of outcrossing in Trillium erectum and Trillium grandiflorum (Liliaceae)
Evidence of outcrossing in Trillium erectum and Trillium grandiflorum (Liliaceae)
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DOI：
10.1093/oxfordjournals.jhered.a023110
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发表时间：
1997-07-01
影响因子：
3.1
通讯作者：
Rogati, P
中科院分区：
文献类型：
--
作者：
Broyles, SB;ShermanBroyles, SL;Rogati, P
采用酶电泳技术研究了延龄草（Trillium erectum）和延龄草（Trillium erectum）的等位酶多样性和异交率。在供试的14个位点中，有11个位点存在多态性，在供试的13个位点中，有7个位点存在多态性。erectum和T.多态位点百分率（P）、平均等位基因数（A）和平均期望杂合度（H-e）在T.直立的（P = 78.6%; A = 2.48; H-e= 0.220）显著高于洋桔梗7号（P = 43.6%; A = 2.18; H-e = 0.1.30），通过固定指数测量，两个物种都没有表现出杂合子的净过剩或不足，这些水平的同工酶多样性在具有异交繁殖系统的物种中比具有自交繁殖系统的物种更典型，通过交配系统分析，发现7号的异交率较高（t（m）= 0.761 +/- 0.076; x +/- SD），而T.直立延龄草是近57%（t（m）= 0.423 +/- 0.093）更典型的一个物种与混合交配系统，然而，这些数据强烈挑战的观点，自交繁殖系统是典型的所有北美物种延龄草。
Enzyme electrophoresis was used to investigate allozyme diversity and outcrossing rates in a population of Trillium erectum and T. grandiflorum from central New York, Enzyme polymorphisms were observed for 11 of 14 and 7 of 13 loci examined in T. erectum and T. grandiflorum, respectively, Percent polymorphic loci (P), mean number of alleles per locus (A), and mean expected heterozygosities (H-e) were greater in T. erectum (P = 78.6%; A = 2.48; H-e= 0.220) than in 7, grandiflorum (P = 43.6%; A = 2.18; H-e = 0.1.30), Neither species showed a net excess nor deficiency of heterozygotes as measured by fixation indices, These levels of allozyme diversity fire more typical of species with outcrossing breeding systems than species with selfing breeding systems, A mating system analysis revealed that outcrossing is high in 7, grandiflorum (t(m) = 0.761 +/- 0.076; x +/- SD), In contrast, the selfing rate in T. erectum was nearly 57% (t(m) = 0.423 +/- 0.093) more typical of a species with a mixed-mating system, Nevertheless, these data strongly challenge the view that selfing breeding systems are typical of all North American species of Trillium.