Mtv-1 superantigen trafficks independently of major histocompatibility complex class II directly to the B-cell surface by the exocytic pathway
Mtv-1 superantigen trafficks independently of major histocompatibility complex class II directly to the B-cell surface by the exocytic pathway
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DOI：
10.1128/jvi.72.4.2577-2588.1998
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发表时间：
1998-04-01
影响因子：
5.4
通讯作者：
Pullen，AM
中科院分区：
文献类型：
--
作者：
Grigg，ME;McMahon，CW;Pullen，AM
将 Mtv-1 超抗原 (vSag1) 呈递给特定的 Vβ 携带 T 细胞需要与主要组织相容性复合物 II 类分子结合。 vSag1 运输到细胞表面的细胞内途径以及抗原呈递 B 淋巴细胞中 vSag1-II 类复合物组装的位点尚未确定。在这里，我们表明 vSag1 通过胞吐分泌途径独立于 II 类运输到质膜。在 B 细胞表面，vSag1 主要与成熟的肽结合 II 类 αβ 二聚体结合，该二聚体在十二烷基硫酸钠中稳定。在没有 II 类分子的情况下，vSag1 在细胞表面不稳定，改变 vSag1 表面表达和 II 类分子构象的试剂会影响 vSag1 对超抗原反应性 T 细胞的刺激。
Presentation of theMtv-1superantigen (vSag1) to specific Vβ-bearing T cells requires association with major histocompatibility complex class II molecules. The intracellular route by which vSag1 trafficks to the cell surface and the site of vSag1-class II complex assembly in antigen-presenting B lymphocytes have not been determined. Here, we show that vSag1 trafficks independently of class II to the plasma membrane by the exocytic secretory pathway. At the surface of B cells, vSag1 associates primarily with mature peptide-bound class II αβ dimers, which are stable in sodium dodecyl sulfate. vSag1 is unstable on the cell surface in the absence of class II, and reagents that alter the surface expression of vSag1 and the conformation of class II molecules affect vSag1 stimulation of superantigen reactive T cells.