Cell adhesion on an artificial extracellular matrix using aptamer-functionalized PEG hydrogels
Cell adhesion on an artificial extracellular matrix using aptamer-functionalized PEG hydrogels
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DOI：
10.1016/j.biomaterials.2011.10.062
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发表时间：
2012-02-01
期刊：
影响因子：
14
通讯作者：
Wang, Yong
中科院分区：
文献类型：
--
作者：
Chen, Niancao;Zhang, Zhaoyang;Wang, Yong
人工细胞外基质（ECM）的开发对再生医学非常重要，因为ECM在细胞行为调节中起着复杂和动态的作用。在这项研究中，核酸适体被应用于功能化水凝胶，用于模拟ECM的粘附位点。结果表明，核酸适体可以通过自由基聚合的方式嵌入到聚乙二醇（PEG）水凝胶中。适体的掺入仅对PEG水凝胶的机械性质产生中等影响。重要的是，结果还表明，适体有效地诱导细胞类型特异性粘附到PEG水凝胶，而不影响细胞活力。细胞粘附是适体浓度、间隔区长度和细胞接种时间的函数。此外，细胞粘附到适体功能化的水凝胶可以通过在生理条件下的适体失活而减弱。因此，适体功能化的水凝胶是用于开发人工ECM的有前途的生物材料。(C)2011爱思唯尔有限公司版权所有。
The development of an artificial extracellular matrix (ECM) is important to regenerative medicine because the ECM plays complex and dynamic roles in the regulation of cell behavior. In this study, nucleic acid aptamers were applied to functionalize hydrogels for mimicking the adhesion sites of the ECM. The results showed that nucleic acid aptamers could be incorporated into polyethylene glycol (PEG) hydrogels via free radical polymerization. The incorporation of the aptamers produced only a moderate effect on the mechanical properties of the PEG hydrogels. Importantly, the results also showed that the aptamers effectively induced cell type-specific adhesion to the PEG hydrogels without affecting cell viability. The cell adhesion was a function of the aptamer concentration, the spacer length and the cell seeding time. In addition, cell adhesion to the aptamer-functionalized hydrogel could be attenuated by means of aptamer inactivation in a physiological condition. Thus, aptamer-functionalized hydrogels are promising biomaterials for the development of artificial ECMs. (C) 2011 Elsevier Ltd. All rights reserved.